甘肃铨水大黄的HPLC指纹图谱研究  

Research on HPLC Fingerprint of Rheum palmatum from Gansu Province Called “Quan-shui-da-huang”

作　　者：钮正睿[1] 刘洪宇[2] 张庆生[1] 曹进[1] 毛淑杰[3] 李先端[3] 

机构地区：[1]中国食品药品检定研究院食品化妆品检定所,北京100050 [2]国家食品药品监督管理总局保健食品审评中心,北京100070 [3]中国中医科学院中药研究所,北京100700

出　　处：《中药材》2013年第4期544-549,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:建立甘肃铨水大黄药材HPLC指纹图谱分析方法,为铨水大黄的质量控制提供依据。方法:用Cosmosil 5C18-PAQ(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%乙酸水溶液为流动相进行梯度洗脱,流速:1 mL/min,柱温:30℃,检测波长:280 nm;测定收集的21份大黄样品,对数据进行相似度评价、主成分分析和聚类分析。结果:建立了铨水大黄HPLC指纹图谱分析方法;根据相似度评价、主成分分析和聚类分析结果,筛选10份样品建立了铨水大黄的特征指纹图谱。结论:在建立的HPLC条件下各成分分离较好,可用于铨水大黄的分析和质量控制。Objective：To establish an analysis method for HPLC fingerprint of Rheum palmatum collected from Gansu province （called ＂Quan-shui-da-huang＂ ） ,and provide basis for quahty control of Quan-shui-da-huang. Methods： The Cosmosil 5C18-PAQ column （250 mm×4. 6 mm,5μm） was used with the mixture of 0. 1% acetic acid and acetonitrile as mobile phase in a gradient elution mode. The flow rate was 1 mL/min, column temperature was 30℃ and detection wavelength was set at 280 nm. The similarity evaluation, principal component analysis （PCA）and cluster analysis of crude drugs coilected from different habitats were carried out. Results： The analysis method of HPLC fingerprint was set up. The characteristic fingerprint was obtained by chemometrics methods. Conclusion ： Under the selected chromatographic conditions, constituents in Rheum palmatum can be separated well. This method can be used for the quality control of Quan-shui-da-huang.

关 键 词：铨水大黄 HPLC指纹图谱 相似度评价 主成分分析 聚类分析 

分 类 号：R282.5[医药卫生—中药学]