白花丹醌对人肝星状细胞肿瘤坏死因子-α、血小板衍化生长因子BB表达的影响  

Effects of Plumbagin on Expression of TNF-α and PDGF-BB in Human Hepatic Stellate Cells Activated by Leptin

作　　者：刘雪梅[1] 赵铁建[1] 彭岳[1] 段雪琳[1] 韦燕飞[1] 

机构地区：[1]广西中医药大学基础医学院生理教研室,广西南宁530001

出　　处：《中药材》2013年第4期594-597,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(81160553;81260675);广西高等学校优秀人才资助项目(J11065);广西教育厅科研课题(200103YB083)

摘　　要：目的:研究白花丹醌对瘦素(Leptin)刺激人肝星状细胞(HSC-LX2)肿瘤坏死因子(TNF-α)、血小板衍化生长因子BB(PDGF-BB)表达的影响。方法:体外培养HSC-LX2,瘦素刺激24 h,各药物与细胞共孵育24 h后,采用荧光实时定量PCR检测各组TNF-αmRNA、PDGF-BB mRNA的表达,免疫印迹法测定各组TNF-α和PDGF-BB蛋白表达情况。结果:白花丹醌作用24 h后,与模型组比较,HSC-LX2细胞中TNF-αmRNA和PDGF-BB mRNA的表达水平显著降低。免疫印记结果显示白花丹醌作用24 h后,HSC-LX2PDGF-BB蛋白表达较模型组显著降低(P<0.01)。结论:白花丹醌抑制HSC-LX,TNF-α、PDGF-BB mRNA和抑制PDGF-BB蛋白水平的表达可能是其抗肝纤维化的主要机制之一。Objective：To investigate the effect of plumbagin on the expression of TNF-a and PDGF-BB in human hepatic stellate cells（ HSC-LX2 ）activated by Leptin. Methods：HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-a mRNA and PDGF-BB mRNA were determined by Real- time quantitative PCR, the protein expressions of TNF-a and PDGF-BB were determined by Western blotting. Results ： The expressions of TNF-a mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group（ P 〈 0. 01 ）, and Western blotting analyses revealed similar trends in protein expression. Conclusion：Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-a and PDGF-BB and the protein of PDGF-BB.

关 键 词：白花丹醌 肝星状细胞 瘦素 肿瘤坏死因子-Α 血小板衍化生长因子BB 

分 类 号：R285.5[医药卫生—中药学]