SPF雏鸡感染REV后IL-6和IFN-γ mRNA表达的动态变化  

Dynamic changes of IL-6 and IFN-γ mRNA expressions in SPF chickens infected with REV

作　　者：陈雪锋[1] 李恩扩[1] 王肖祎[1] 陈静[1] 胡敬东[1] 

机构地区：[1]山东农业大学动物医学院山东省动物生物工程与疾病防治重点实验室,山东泰安271018

出　　处：《中国兽医杂志》2013年第6期10-13,共4页Chinese Journal of Veterinary Medicine

基　　金：山东省自然科学基金项目资助(Y2008D12)

摘　　要：建立并利用鸡白细胞介素6和γ干扰素荧光定量RT-PCR方法,对于禽网状内皮组织增生症病毒(Reticuloendo-theliosis virus,REV)感染SPF鸡后主要免疫器官的鸡白细胞介素6和γ干扰素基因转录水平进行了初步研究。结果表明,与对照组相比,处理组脾脏、胸腺和腔上囊中IL-6和IFN-γ的分泌水平在所有时间点均显著升高(P<0.05)或极显著升高(P<0.01)。本试验为探讨REV感染的免疫抑制机理打下了一定基础。In this study, a real-time reverse-transcription PCR was developed for quantitative analysis of chicken interleukin 6 and interferon-γmRNA and then the expression levels of interleukin 6 and interferon-γmRNA in the primary immune organs of SPF chickens infected with reticuloendotheliosis virus （REV）were investigated using this method. The results showed that. IL-6 and IFN-γgene expression levels were significantly higher （P〈0.05） at all tested time points in the spleen, thymus and bursa of Fabricius in SPF chickens from treatment group than those in the control group. Our study laid the foundation for investigating the mechanism of immunosuppression induced by REV.

关 键 词：禽网状内皮组织增生症病毒 白细胞介素6 Γ干扰素 实时荧光定量RT-PCR 

分 类 号：S852.659.3[农业科学—基础兽医学]