对虾白斑症病毒的初步研究  

Primary studies on white spot syndrome virus of penaeid shrimp

作　　者：刘凯于[1] 余泽华[1] 杨凯[2] 王家坤[1] 虢华珊[1] 姚汉超[1] 陈曲侯[1] 徐敬明[3] 

机构地区：[1]华中师范大学生命科学学院昆虫学研究所,武汉430079 [2]中山大学生命科学院 [3]临沂师范学院生物系,山东临沂276005

出　　处：《华中师范大学学报（自然科学版）》2000年第3期336-340,共5页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省自然科学基金资助项目! ( 952 3 )

摘　　要：对虾白斑症病毒可通过人工投喂感染克氏螯虾 ,病程发展迅速 ,死亡率高 .该病毒既可见在胞核中复制 ,也常见于胞质中装配 .通过差速离心 ,蔗糖垫层离心或密度梯度离心后 ,再将分离物中细胞源核酸消化掉 ,可提取出较纯的病毒 DNA.White spot syndrome viruses could infect Cambarus clarkii by feeding them. The disease developed rapidly with high mortality. The viruses were assembled in nuclei or cytoplasm. They could be separated roughly by different centrifugation, sucrose cushion centrifugation or density gradient centrifugation, then the nucleoacid orgined from cells in rough viruses was digested, and pure viral DNA was obtained.

关 键 词：白斑症病毒 组织病理 投喂感染 形态学 对虾 

分 类 号：S945.469[农业科学—水产养殖]