煤烘高氟玉米染毒大鼠的血清TRACP-5b表达变化  

Expression Variation of Serum TRACP-5b in Rats Treated with Coal-Burning Fluorine

作　　者：韦艳[1] 张震[1] 刘新儒[1] 张华[1] 

机构地区：[1]贵阳医学院公共卫生学院环境卫生学教研室,贵阳550004

出　　处：《环境与职业医学》2013年第7期520-523,共4页Journal of Environmental and Occupational Medicine

基　　金：科技部国际合作项目(编号:2010DFB30530);贵州省科学技术基金(编号:黔科合J字[2010]2268号)

摘　　要：[目的]观察煤烘高氟玉米染毒大鼠的血清抗酒石酸酸性磷酸酶5b(TRACP-5b)的表达变化,探讨骨吸收在氟骨症发生机制中的作用。[方法]将断乳4周的SD大鼠120只随机分为4组,对照组给予商品化饲料,3个实验组在对照饲料基础上给予不同比例煤烘高氟玉米,低、中、高剂量染毒组饲料含氟量分别为9.56、15.89及23.00mg/kg。于连续染毒至第30、90、180天时,检测大鼠骨氟、尿氟含量及氟斑牙发生率、股骨病理组织学积分,ELISA法检测血清TRACP-5b水平。[结果](1)各剂量组骨氟、尿氟、氟斑牙发生率及股骨病理学积分均随着染毒剂量的增加而升高(P<0.05)。染毒至第30、90、180天时,各剂量组的骨氟、尿氟均高于对照组(P<0.05),且剂量较高的组别的尿氟、骨氟高于剂量较低的组别(P<0.05)。染毒至第90天和180天时,各剂量组氟斑牙发生率高于对照组(P<0.05);中、高剂量组股骨病理切片积分高于低剂量组和对照组(P<0.05)。(2)各组染毒至180天时,血清TRACP-5b均高于同剂量组90天及30天时的水平(P<0.05)。在第30天及90天时,各组血清TRACP-5b差异无统计学意义;染毒至180天时,高剂量组TRACP-5b高于对照组和中剂量组(P<0.05),其余各组间差异无统计学意义。[结论]摄入煤烘高氟玉米剂量较高,且摄入时间较长时,可能引起血清TRACP-5b表达升高。[Objective]To observe the expression variation of serum tartrate-resistant acid phosphatase isoform 5b（TRACP-5b） in rats exposed to coal-burning fluorine,and explore the role of bone resorption in the pathogenesis of skeletal fluorosis.[Methods] One hundred and twenty SD rats weaned for 4 weeks were divided into 4 groups： 1 control group was fed with commercial feed,and the other 3 experimental groups were fed with mixed feeds which contained 9.56mg/kg（low-dose group）,15.89mg/kg（mediumdose group）,and 23.00mg/kg（high-dose group） of fluorine.The animals were executed after 30d,90d,and 180d of treatment to determine bone and urinary fluoride content,incidence rate of dental fluorosis,and femur pathological score.TRACP-5b levels in serum were tested by ELISA.[Results]（1） The bone and urinary fluoride concentrations,the dental fluorosis incidences,and the femur pathological scores of the 3 experimental groups were significantly increased in a dose-dependent manner respectively（all P 〈0.05）.After the treatment for 30d,60d,and 90d,the urinary and bone fluoride concentrations of each experimental group were statistically higher than the control group（all P 〈0.05）,and those of the higher dose group were remarkably higher than those of the lower dose group respectively（all P 〈0.05）.For 90d and 180d,the incidence of dental fluorosis in each dose group was obviously higher than that of controls（all P 〈0.05）;the femur pathological score of the medium-dose and the high-dose groups were significantly higher than that of the low-dose group and the controls（all P 〈0.05）.（2） The serum TRACP-5b levels in each experimental group with 180d of exposure were statistically higher than those in the same dose group with 30d and 90d of exposure（all P 〈0.05）.There was no significant difference in the serum TRACP-5b among the groups after treatment for 30d and 90d.After the treatment for 180d,the TRACP-5b levels of the high-dose group was significantly higher than that of the cont

关 键 词：燃煤型氟中毒 抗酒石酸酸性磷酸酶5B 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]