系统性红斑狼疮患者外周血CD4^+和CD8^+T细胞PD-1的表达和意义  

Expression and significance of PD-1 on CD4^+ and CD8^+ T cells from the peripheral blood of patients with systemic lupus erythematosus

作　　者：王元元[1] 谢长好[2] 孙美群[1] 柴继侠[1] 周艳梅[1] 陈勇[3] 唐洁[3] 李柏青[3] 

机构地区：[1]蚌埠医学院组织学与胚胎学教研室,安徽蚌埠233030 [2]蚌埠医学院第一附属医院风湿免疫科,安徽蚌埠233030 [3]蚌埠医学院感染与免疫省重点实验室,安徽蚌埠233030

出　　处：《细胞与分子免疫学杂志》2013年第9期962-965,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省卫生厅青年基金(09B108);蚌埠医学院科研项目(BY1003)

摘　　要：目的探讨程序性死亡分子1(PD-1)在系统性红斑狼疮(SLE)患者外周血CD4^+和CD8^+T细胞上的表达及临床意义。方法应用流式细胞仪检测51例SLE患者和38例健康对照者外周血T细胞亚群表面PD-1表达水平,比较SLE稳定组、活动组和健康对照组以及狼疮肾炎组和无狼疮肾炎组之间CD4^+和CD8^+T细胞表面PD-1表达的百分比,并分析其与临床表现及实验室检查数据的相关性。结果 SLE活动组CD4^+T细胞PD-1表达水平高于健康对照组和不活动组,差异均有统计学意义(P<0.05)。SLE活动组、稳定组CD8^+T细胞PD-1表达水平均高于健康对照组,差异有统计学意义(P<0.05)。狼疮肾炎患者CD4^+PD-1^+和CD8^+PD-1^+T细胞分别高于无狼疮肾炎患者(P<0.01)。SLE患者中抗dsDNA抗体、抗Sm抗体、抗核小体抗体阳性组外周血CD4^+和CD8^+T细胞PD-1表达水平均高于对应阴性组。SLE患者CD4^+和CD8^+T细胞PD-1表达百分率与SLE疾病活动度指数(SLEDAI)、尿蛋白定量呈正相关,与补体C3呈负相关。结论 SLE患者外周血CD4^+和CD8^+T细胞PD-1表达异常,与SLEDA1和自身抗体产生有明确的相关性。Objective To detect the expression of programmed death-1 （PD-I） on CD4 ＋ and CD8 ＋ T cells from the peripheral blood of patients with systemic lupus erythematosus （SLE） and analyze its clinical implications. Methods The expression of PD-1 on the CD4 ＋ and CD8 ＋ T cells were examined in 51 SLE patients and 38 healthy controls （HCs） by flow cytometry. The proportions of PD-1 positive CD4 ＋ and CD8 ＋ T cells were compared not only between inactive or active SLE patients and HCs, but also between patients with and without lupus nephritis. The correlations of PD-1 expression with clincal manifestations and laboratory findings were analyzed. Results The proportions of CD4 ＋ PD-1 ＋ T cells significantly increased in active SLE patients as compared with HCs and inactive SLE ones （all P〈0.05）. The proportions of CD8＋ PD-I ＋ T cells were significantly higher in active and inactive SLE patients than those in HCs （all P 〈 0.05）. The numbers of CD4 ＋ PD-I ＋ and CD8 ＊ PD-I ＋ T cells in SLE patients with nephritis were significantly higher than those in patients without nephritis （ P 〈 0.01）. In SLE patients, the numbers of CD4 ＋ PD-1 ＋ and CD8 ＋ PD-1 ＋ T cells were significantly higher in ones with positive anti-dsDNA, anti-Sm, and anti-nucleosome antibodies than in corresponding negative ones; the proportions of CD4 ＋ PD-1 ＋ and CD8 ＋ PD-1 ＋ T cells were positively correlated with SLEDAI score and the amount of proteinuria, but were negatively correlated with complement C3. Conclusion The aberrations of CD4 ＋ PD-1 ＋ and CD8 ＋ PD-1 ＋ T cells were observed in patients with SLE. Increased numbers of CD4 ＋ PD-1 ＋ and CD8 ＊ PD-1 ＋ T cells were correlated with increased disease activity and the production of anti-self antibodies,

关 键 词：系统性红斑狼疮 PD-1 CD4+T细胞 CD8+T细胞 

分 类 号：R593.241[医药卫生—内科学] R392-33[医药卫生—临床医学]