三七总甙对增生性瘢痕的作用  

Notoginseng glycosides effects on hyperplastic scar

作　　者：刘凯[1] 潘亮亮[1] 李婷[1] 刘树发[1] 

机构地区：[1]佳木斯大学附属第二医院(口腔医院)颌面外科,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2013年第24期4436-4443,共8页Chinese Journal of Tissue Engineering Research

摘　　要：背景:增生性瘢痕的传统治疗手段如手术切除、类固醇激素、抗代谢药、免疫抑制剂和放射疗法等,均易复发或有严重的不良作用而限制了其临床应用。近年来应用活血化瘀类中药提取成分治疗增生性瘢痕取得了较为理想的进展,而且不良反应小。目的:通过组织染色及相关基因检测的方法,观察三七总甙对兔耳增生性瘢痕的作用以及对转化生长因子β1mRNA表达的影响。方法:创建兔耳增生性瘢痕模型,约术后4周,按随机数字表法将24只大耳白兔分为3组,三七总甙组、曲安奈德组及空白对照组每组8只。以曲安奈德为阳性对照,分组分次瘢痕基底局部给药,用药5次后取瘢痕组织,以苦味酸酸性复红染色法、苏木精-伊红染色法对胶原纤维、成纤维细胞进行观察,应用反转录PCR方法检测细胞内转化生长因子β1基因的表达情况。结果与结论:苏木精-伊红染色结果示三七总甙组兔耳瘢痕中成纤维细胞数量低于空白对照组,与曲安奈德组无明显差异。苦味酸酸性复红染色结果示三七总甙组兔耳瘢痕中胶原纤维较空白对照组排列整齐,与曲安奈德组无明显差异。基因检测结果显示三七总甙组、曲安奈德组转化生长因子β1mRNA的表达显著低于空白对照组(P<0.05)。提示三七总甙及曲安奈德均能通过抑制转化生长因子β1mRNA的表达,降低转化生长因子β1在瘢痕组织中的含量,从而抑制成纤维细胞的过度增殖及以胶原为主的细胞外基质过度沉积,进而降低胶原纤维合成,来达到有效抑制瘢痕组织过度增生的目的。BACKGROUND： Traditional treatments for hypertrophic scars, such as surgical resection, steroid hormones, antimetabolite, immunosuppressants and radiotherapy, are prone to resulting in relapse or serious adverse effects that limit their clinical application. In recent years, extraction of Chinese medicine for invigorating blood circulation and eliminating stasis has made satisfactory progress in the treatment of hypertrophic scars, and causes little adverse reactions. OBJECTIVE： To observe the effect of notoginseng glycosides on rabbit ear hyperplastic scar and mRNA expression of transforming growth factor beta 1 using histological staining and related gene detection methods. METHODS： A rabbit ear model of hyperplastic scar was established. At 4 weeks after modeling, 24 model rabbits were randomly divided into three groups, eight rabbits in each group： notoginseng glycosides group, triamcinolone acetonide group （positive control group）and blank control group. Local administration was performed in the three groups, five times. Then, scar tissues were extracted and dyed with Van-Gieson staining and hematoxylin-eosin staining for observation of collagen fibers and fibroblasts. Expression of transforming growth factor beta 1 mRNA was detected suing reverse transcription-PCR method. RESULTS AND CONCLUSION： The number of fibroblasts number in the scar was lower in the notoginseng glycosides group than the blank control group, but there was no difference between the triamcinolone acetonide group and notoginseng glycosides group. Collagen fibers arranged more regularly in the notoginseng glycosides group as compared with the blank control group, and there was also no difference between the triamcinolone acetonide group and notoginseng glycosides group. Expression of transforming growth factor beta 1 mRNA was significantly lower in the triamcinolone acetonide and notoginseng glycosides groups than the blank control group （P 〈 0.05）. These findings indicate that notoginseng glycosides and triamcinolone a

关 键 词：组织构建 皮肤组织构建 三七总甙 增生性瘢痕 曲安奈德 转化生长因子β1 反转录PCR 基因 检测 苏木精伊红 苦味酸酸性复红 成纤维细胞 胶原纤维 

分 类 号：R318[医药卫生—生物医学工程]