脊神经前根吻合重建脊髓损伤大鼠的股四头肌功能  

Reconstruction of quadriceps femoris by anatomizing the ventral roots of spinal nerves in rats with spinal cord injury

作　　者：李伟[1] 钟贵彬[2] 

机构地区：[1]济南市第三人民医院脊柱关节科,山东省济南市250101 [2]上海交通大学附属仁济医院骨科,上海市200001

出　　处：《中国组织工程研究》2013年第24期4502-4508,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30801157)~~

摘　　要：背景:如何重建脊髓损伤肢体运动功能对截瘫患者具有重要的意义。目的:探索利用脊髓损伤平面以上健存的脊神经前根与支配股四头肌的腰神经建立神经通路,恢复脊髓损伤后股四头肌的神经支配和肌收缩功能。方法:对清洁级SD大鼠L1神经根的前根与L3神经根的前根进行显微吻合。经一段时间(6个月)的轴突再生后,期望建立新的肌肉收缩功能。神经缝合后6个月,在破坏L2脊髓节段前后,分别进行神经电生理检测,观察股四头肌神经支配情况。结果与结论:在同侧L2半切脊髓前后,电刺激移植神经干时可记录到股四头肌的收缩肌电图。在同侧L2半切脊髓前后,电刺激L1感觉根时可同样在股四头肌记录到肌电图。说明利用脊髓损伤平面以上健存的L1神经根前根与L3神经前根移植吻合能重建新的股四头肌神经支配反射通路,并使股四头肌低级反射中枢上移。BACKGROUND： Reconstruction of motor function of limbs in spinal cord injury is significant for patients with paraplegia. OBJECTIVE： To recover the innervations and muscle contraction function of quadriceps femoris in spinal cord injured rats by constructing the neural pathway using the ventral roots of spinal nerves above injuredsegment of spinal cord and lumbar nerve that dominates the quadriceps femoris METHODS： LI ventral root was anatomized to L3 ventral root from Sprague-Dawley rats. After 6 months, axona regeneration was found from L1 ventral root to L3 ventral root through the grafting nerve, which may reconstruct muscle contraction function. After nerve suture for 6 months, spinal cord injury was made in Lz segment for neuroelectrophysiology examination to observe innervation of quadriceps femoris. RESULTS AND CONCLUSION： After semi-dissection of L2 segment, wave from quadriceps femoris was recorded in electromyogram meter when stimulating the grafting nerve. After injured the segment of L2, we also record wave from quadriceps femoris when stimulating L1 ventral root. Results showed that anastomosis of the L1 ventral roots of spinal nerves above injured segment of spinal cord and the ventral root below the injured spinal cord segment can reconstruct the reflex path of innervation of quadriceps femoris, and the reflex center of quadriceps femoris in the spinal cord moves up to L1 segment.

关 键 词：组织构建 组织构建基础实验 脊神经 脊髓损伤 神经移植 股四头肌 肌肉收缩 肌电图 轴突再 生 反射中枢 SD大鼠 电生理 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]