河南省SIV地方株分离鉴定及生物学特性研究  

Isolation , identification and biological characterizations of swine influenza virus in Henan Province

作　　者：岳耀辉[1,2] 菅复春[1,2] 胡慧[1,2] 张云[1,2] 张龙现[1,2] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南省人兽共患病国际联合实验室,郑州450002

出　　处：《中国人兽共患病学报》2013年第7期659-664,670,共7页Chinese Journal of Zoonoses

基　　金：国家科技支撑计划课题(2010BAD04B01);河南省高校科研创新团队(012IRTSTHN005)资助~~

摘　　要：目的进一步阐明河南省猪场猪流感病毒生物学特性。方法用鸡胚法从河南某大型屠宰场随机采集的360份猪鼻拭子和某猪病门诊所采集有呼吸道症状的35份猪鼻拭子和肺脏脾脏样品里分离猪流感病毒。结果成功分离到3株猪流感病毒,分别命名为:A/Swine/Henan/01/2012(H1N2),A/Swine/Henan/01/2012(H1N1),A/Swine/Henan/02/2012(H1N1)。这3株病毒的生物学特性研究结果表明分离株对鸡、兔、小白鼠及牛1%红细胞都有血凝性,该分离株对乙醚、氯仿和pH3.0的盐酸这3种化学试剂敏感,并且不同毒株EID50、ELD50有所差异,感染小鼠第4d开始发病。结论河南省猪场有不同亚型流感病毒感染,应进一步研究和关注。To further understand the prevalence of swine influenza in pig farm in Henan province, 360 porcine nasal swabs and 35 swine lung and spleen samples of pigs that showed clinic signs of respiratory symptoms were collected from one big slaughterhouse and one clinic service of swine diseases in Henan Province. The chicken embryo inoculation method was used for virus isolation, the biological characterizations of swine influenza virus were tested and analysed. The results showed that three strains of swine influenza virus were isolated successfully, two isolates were H1N1 subtype and one was H1N2 subtype, named as： A/Swine/Henan/01/2012（H1N2）, A/Swine/Henan/01/2012（H1N1） and A/Swine/Henan/02/2012（H1N1） respectively. On this basis, these strains isolated were purified by limited dilution cloning. Biological characteristics research showed： the isolates had hemagglutinin to 1% red cells of chicken, rabbit, mouse and bovine respectively. In addition, the isolates were sensitive to the chemical reagent of Ethyl ether, chloroform and acids of pH3.0. And the different strains of EID50 and ELD50 were different biological characteristics with each other, infection of mice were ill after the onset of 4 days. The swine influenza is a epidemic disease in Henan, and zoonotic subtype exist in pig farm, the influence to public health need to take more attention.

关 键 词：猪流感 H1N1 H1N2 分离 鉴定 生物学特性 

分 类 号：S855.3[农业科学—临床兽医学]