丹栀逍遥散防治大鼠多囊卵巢综合征的蛋白质组学研究  

Proteomic analysis of Danzhixiaoyao Powder on prevention of polycystic ovary syndrome in rats

作　　者：张丽华[1,2] 靖林林[1,2] 黄璜[1,2] 孙学刚[1,2] 刘颖[3] 

机构地区：[1]南方医科大学中西医结合医院,广州510315 [2]南方医科大学中医药学院,广州510315 [3]广东省人民医院,广州510080

出　　处：《中药药理与临床》2013年第1期1-4,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173168);广东省医学科学技术研究基金(A2010036)

摘　　要：目的:观察丹栀逍遥散对多囊卵巢综合症(PCOS)大鼠差异蛋白质表达谱的影响。方法:应用皮下埋植左旋18-甲基炔诺酮硅胶棒联合人绒毛膜促性腺激素注射复制PCOS模型;双向电泳技术分离差异蛋白表达谱,基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)鉴定差异蛋白点;免疫组化、蛋白免疫印迹和荧光定量PCR验证结果。结果:丹栀逍遥散3.75g/kg显著改善大鼠卵巢增大及卵泡扩张,质谱成功鉴定8个蛋白点,其中点5为β抑制蛋白2(β-arrestin 2,β-arr2),β-arr2在模型组显著降低,丹栀逍遥散显著增加其表达(P<0.01)。结论:丹栀逍遥散通过增加β-arr2表达,调控β-arr2介导的信号通路防治PCOS。Objective： To identify the underlying mechanisms in the protective effects of Danzhixiaoyao Powder（DZXYP） on polycystic ovary syndrome rat model.Methods： PCOS was introduced by subcutaneous implantation of 18-methyl norethisterone silica gel stick combined with subcutaneous injection of human chorionic gonadotrophin.Two-dimensional polyacrylamide gel electrophoresis（2-DE） and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS） were used to identify differentially expressed proteins.Immunohistochemistry,real time quantitative polymerase chain reaction（RQ-PCR） and western blot were performed to verify the results above.Results： DZXYP ameliorated ovary enlargement and follicle dilatation.Among eitht successfully identified proteins that were differentially expressed,β-arrestin 2（β-arr2） was upregulated in DZXYP pretreated PCOS group when compared with PCOS group.Conclusion： DXR could alleviate PCOS which might be related to increased expression of β-arr2.

关 键 词：丹栀逍遥散 多囊卵巢综合征 蛋白质组学 β-arrestin2 

分 类 号：R285[医药卫生—中药学]