脑复聪对脂多糖激活的小胶质细胞合成环氧合酶-2和分泌前列腺素E_2作用的实验研究  

Experimental Study on the Effects of Naofucong on Lipopolysaccharide-activated Synthesis of Cyclooxygenase-2 and Secretion of Prostaglandin E_2 by Microglia

作　　者：崔雅忠[1] 景光婵[1] 张孟仁[1] 刘玉琴[2] 顾蓓[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院中医科,100730 [2]中国医学科学院基础医学研究所

出　　处：《医学研究杂志》2013年第7期101-104,共4页Journal of Medical Research

摘　　要：目的观察中药脑复聪(NFC)含药血清对脂多糖(LPS)激活后的小鼠神经小胶质细胞BV-2的形态、BV-2中环氧合酶-2(COX-2)蛋白的表达以及BV-2上清液中前列腺素E2(PGE2)分泌量水平的影响。方法将体外培养的小鼠神经小胶质细胞BV-2分成:正常对照组、LPS组、NFC血清组(高、中、低剂量)培养24h,用倒置相差显微镜观察细胞形态,蛋白印迹方法检测BV-2中COX-2蛋白的表达,酶联免疫吸附法测定培养BV-2上清液中PGE2蛋白的浓度。结果 (1)NFC各组对LPS所致的BV-2细胞形态变化均有一定的恢复作用。(2)LPS组BV-2中COX-2含量较正常对照组升高;与LPS组相比,NFC含药血清各剂量组COX-2蛋白含量均下降。(3)BV-2上清液中PGE2蛋白的浓度LPS组较正常对照组含量升高;与LPS组相比,NFC各剂量组PGE2含量均下降。结论 NFC含药血清可以显著下调LPS激活后BV-2中COX-2蛋白的表达量并显著减少BV-2上清液中PGE2的分泌量,减轻炎症反应,改善细胞形态。Objective To observe the effects of Naofucong serum on morphology of the mouse microglia BV - 2, expression of COX - 2 protein in BV - 2, and the level of PGE2 secreted in the supernatant of the BV - 2 cuhure after lipopolysaccharide activation. Methods Mouse microglia BV -2 cells incubated in vitro were divided into three groups：the normal control group,the LPS group and the high - , moderate - and low - dose of NFC serum groups. After 24h incubation,the morphology of microglia was observed with inverted phase con- trast microscope, and the expression of the COX -2 protein was detected by Western blot, and the level of PGE2 protein in the supernatantof BV - 2 culture was determined by enzyme - linked immunosorbent assay. Results （ 1 ） Each concentration of NFC - containing serum group had a certain restoring effect on the LPS - induced morphological changes. （2） The level of COX - 2 protein in BV - 2 in the LPS group was significantly increased compared to the control group ; while the levels of COX - 2 protein in BV - 2 in all of the various doses of NFC - containing serum groups were decreased compared to the LPS group. （3） The culture supernatant of BV - 2 in the LPS group ex- hibited significantly increased level of PGE2 compared to the normal control group; while all of the various doses of NFC - containing ser- um groups exhibited decreased PGE2 level compared to the LPS group. Conclusions The NFC - containing serum is able to significantly down regulate the expression of COX - 2 protein in BV - 2 activated by LPS and reduce the secretion of PGE2 in the supernatant of BV - 2 culture. Inflammation reaction can be relieved while morphology of cells can be improved.

关 键 词：脂多糖 小胶质细胞 环氧合酶2前列腺素E2 脑复聪 

分 类 号：R719.31[医药卫生—妇产科学]