MDR1-targeted siRNA delivery with cationic dendritic conjugated polymers  

作　　者：FENG XuLi Lü FengTing LIU LiBing WANG Shu 

机构地区：[1]Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2013年第22期2762-2766,共5页

基　　金：supported by the National Natural Science Foundation of China(21203213,21033010,21021091)

摘　　要：A cationic dendritic polyfluorene (PFP) is examined as a siRNA delivery vector. This material was designed to facilitate the nucleic acid binding, encapsulation and efficient cellular uptake. PFP can effectively protect siRNA against nuclease degradation, which is necessary for gene carriers. PFP can be used for multidrug resistance gene-targeted siRNA delivery in doxorubicin (Dox)-resistant human breast cancer cells (MCF7) cells. As a siRNA transfection agent, PFP can efficiently achieve the reversal of drug resistance and enhance the drug sensitivity. These new features and capabilities represent a major step toward conjugated polymers that can function for therapeutic application.A cationic dendritic polyfluorene （PFP） is examined as a siRNA delivery vector. This material was designed to facilitate the nu- cleic acid binding, encapsulation and efficient cellular uptake. PFP can effectively protect siRNA against nuclease degradation, which is necessary for gene carriers. PFP can be used for multidrug resistance gene-targeted siRNA delivery in doxorubicin （Dox）-resistant human breast cancer cells （MCFT） cells. As a siRNA transfection agent, PFP can efficiently achieve the reversal of drug resistance and enhance the drug sensitivity. These new features and capabilities represent a major step toward conjugated polymers that can function for therapeutic application.

关 键 词：siRNA 共轭聚合物 阳离子 MDRL 树突 传递 多药耐药基因 人乳腺癌细胞 

分 类 号：Q78[生物学—分子生物学]