替比夫定单药或联合干扰素体外对SH-SY5Y细胞系线粒体的影响  

Effects of telbivudine alone or in combination with interferon on the mitochondria of SH-SY5Y cell lines in vitro

作　　者：韩丽娜[1] 于岩岩[1] 

机构地区：[1]北京大学第一医院感染疾病科,100034

出　　处：《传染病信息》2013年第3期171-174,共4页Infectious Disease Information

基　　金：国家"十二五"科技重大专项(2012ZX10002-003)

摘　　要：目的研究单用替比夫定(LdT)或联合干扰素(interferon,IFN)对人神经母细胞瘤细胞SH-SY5Y细胞系的线粒体超微结构和线粒体DNA(mitochondrial DNA,mtDNA)含量的影响。方法用CCK-8细胞毒性检测试剂盒检测单用LdT或联合IFN对细胞活力的影响;用透射电镜(transmission electron microscopy,TEM)观察药物对细胞线粒体超微结构的影响;用实时荧光定量PCR法研究分析药物对细胞mtDNA含量的影响。结果 LdT>1000μmol/L时能够显著降低细胞活力(P<0.05),随着处理时间延长细胞活力逐渐降低。在TEM下,在单用LdT或与IFN联合处理的细胞中,未发现有线粒体数量和超微结构的变化。实时荧光定量PCR法结果提示,随着LdT浓度增加,mtDNA含量未下降,并且与IFN联合处理的细胞mtDNA含量也未降低。结论单用LdT或与IFN联合应用不能改变线粒体超微结构以及抑制mtDNA合成。Objective To investigate the effects of telbivudine （LdT） alone or in combination with interferon （IFN） on the mito- chondrial ultrastructure and the content of mitochondrial DNA （mtDNA） of the human neuroblastoma cell line SH-SYSY. Methods Cell cytotoxicity counting kit-8 （CCK-8） was used to analyze the effects of LdT alone or in combination with IFN on the cell viability. Transmission electron microscopy （TEM） was used to observe the effects of drugs on the mitochondrial uhrastructure. Quantitative real-time PCR was used to analyze the effects of drugs on the content of mtDNA. Results When the concentration of LdT was greater than 1000 μmol/L, the cell viability was inhibited significantly （P〈0.05）, and as the treatment was prolonged, the cell viabili- ty decreased gradually. Under the observation of TEM, the number and ultrastructure of mitochondria in cells treated with LdT alone or in combination with IFN didn＇t change. The results of quantitative real-time PCR showed that the content of mtDNA didn＇t de- crease as the cells were treated either with increased concentration of LdT alone or in combination with IFN. Conclusion LdT alone or in combination with IFN can not change mitochondrial uhrastructure and inhibit mtDNA synthesis.

关 键 词：抗病毒药 干扰素类 神经母细胞瘤 细胞系 DNA 线粒体 

分 类 号：R730.264[医药卫生—肿瘤]