正加速度暴露下急性胃黏膜损伤胃组织中MDA、SOD水平的变化  

Levels of MDA and SOD in acute gastric mucosal injury in rats exposed to positive acceleration

作　　者：邵颖锬[1] 李静[2] 陈英[2] 杨春敏[2] 范勤[2] 唐合兰[2] 贾敏[2] 王建昌[2] 辛益妹[3] 

机构地区：[1]河北北方学院,河北省张家口市075000 [2]中国人民解放军空军总医院干部病房,北京市100142 [3]中国人民解放军空军航空医学研究所,北京市100142

出　　处：《世界华人消化杂志》2013年第18期1733-1739,共7页World Chinese Journal of Digestology

基　　金：中国人民解放军全军十二五科技攻关基金资助项目;No.AKJ11J004~~

摘　　要：目的:研究正加速度(+Gz)暴露下急性胃黏膜损伤胃组织中氧自由基代谢指标丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)的水平变化,阐明+Gz暴露对胃黏膜损伤的影响,并研究氧自由基在其中的作用,为飞行员胃肠病的防治提供理论依据.方法:30只♂SD大鼠随机分成以下3组:A组为无水乙醇对照组,B组为无水乙醇+5Gz值暴露组,C组为无水乙醇+10Gz值暴露组.每组大鼠各10只,适应性喂养10d后禁食24h,禁水12h,用无水乙醇(0.4mL/100mg)灌胃1h后,A组不受加速度作用,B、C组分别于+5Gz、+10Gz值下连续暴露3min.每组下离心机后立即予戊巴比妥麻醉取大鼠胃组织,观察各组胃黏膜损伤情况及光镜观察组织形态学的改变,按GUTH法及Whittle法计算胃黏膜损伤指数,并用ELISA法检测胃黏膜中MDA、SOD的含量.结果:(1)各组大鼠胃黏膜在肉眼及光镜下观察均有损伤,损伤程度:C组>B组>A组,A组(肉眼11.410±3.742,光镜3.800±1.399),B组肉眼可见胃黏膜充血、水肿,散在出血斑点,光镜下可见急性炎细胞浸润(肉眼23.654±9.678,光镜5.000±1.054),与A组相比差异有统计学意义(P<0.05);C组胃黏膜损伤最重,肉眼可见胃黏膜弥漫性充血、水肿,伴糜烂、大面积出血斑,光镜下可见大部分腺体结构紊乱,组织间质片状充血、糜烂,急性炎细胞浸润(肉眼49.080±10.254,光镜9.400±2.011),与A、B两组相比差异均有统计学意义(P<0.05);(2)与A组相比,B组胃黏膜中MDA含量升高不明显(0.255±0.074vs0.235±0.044),差异无统计学意义(P>0.05),C组胃黏膜中MDA含量明显升高(0.376±0.084vs0.235±0.044),与A、B两组相比差异均有统计学意义(P<0.05);与A组相比,B组胃黏膜中SOD含量下降不明显(10.000±1.067vs10.694±0.965),差异无统计学意义(P>0.05),C组胃黏膜中S O D含量明显下降(8.852±1.001vs10.694±0.965),与A、B两组相比差异均有统计学意义(P<0.05).结论:+Gz值暴露可加重急性胃黏膜�AIM： To determine the levels of malondialdehyde （MDA） and superoxide dismutase （SOD） in acute gastric mucosal injury in rats exposed to positive acceleration （＋Gz）, observe the impact of ＋Gz exposure on gastric mucosal injury, and clarify the role of oxygen free radicals in this process. METHODS： Thirty male SD rats were randomly divided into three groups： A （ethanol）, B （ethanol with ＋5Gz exposure）, and C （ethanol with ＋10Gz exposure）. All rats were intragastrically given ethanol （0.4 mL/100 mg） after 24 h of fasting and water deprivation for 12 h. One hour after ethanol administration, group A did not undergo ＋Gz, while groups B and C were continuously exposed to ＋5Gz and ＋10Gz for 3 minutes, respectively. Immediately after ＋Gz exposure, gastric tissue samples were taken to observe gastric mucosal injury by light microscopy. The indexes of gastric mucosal injury were calculated, and the contents of MDA and SOD in the gastric mucosa were determined by ELISA. RESULTS： Gastric mucosal injury was observed in all the groups, and gastric mucosal injury was most severe in group C, followed by groups B and A. Group A had mildest gastric mucosal injury. Group B showed visible gastric mucosal congestion, edema and scattered bleeding spots, and light microscopy revealed acute inflammatory cell infiltration. The indexes of gastric mucosal injury were significantly higher in group B than in group A （naked eye： 23.654 ± 9.678 vs 11.410 ± 3.742; light microscopy： 5.000 ± 1.054 vs 3.800 ± 1.399; both P 0.05）. Group C had heaviest gastric mucosal injury. Diffuse gastric mucosal congestion, edema, erosion and many bleeding spots were visible, and light microscopy revealed disorderly arranged gland structure, interstitial congestion, erosion, and acute inflammatory cell infiltration. The indexes of gastric mucosal injury were significantly higher in group C than in groups A and B （naked eye： 49.080 ± 10.254, light microscopy： 9.400 ± 2.011; all P 0.05）.

关 键 词：正加速度 氧自由基 丙二醛 超氧化物歧化酶 胃 黏膜 

分 类 号：R85[医药卫生—航空、航天与航海医学]