基因芯片分析弓形虫感染宿主细胞microRNAs差异表达  

Differential expression of host microRNAs in Toxoplasma gondii infection analyzed by microarray

作　　者：李春香[1] 郝振华[1] 兰兰[1] 华倩倩[1] 梁韶晖[1] 

机构地区：[1]温州医科大学寄生虫学教研室,浙江温州325035

出　　处：《温州医学院学报》2013年第7期430-434,共5页Journal of Wenzhou Medical College

基　　金：浙江省自然科学基金资助项目(Y2100971)

摘　　要：目的:研究弓形虫感染人包皮成纤维细胞(HFFs)的microRNAs(miRNAs)差异表达。方法:基因芯片分析弓形虫感染的HFFs的miRNAs差异表达,并用实时荧光定量RT-PCR法和Northern blot法验证miRNAs差异表达。结果:弓形虫感染HFFs的46种miRNAs表达量有1倍以上上调,37种miRNAs表达量有1倍以上下调。其中4种差异表达的miRNAs的实时荧光定量RT-PCR法和Northern blot法验证结果与基因芯片分析结果一致。结论:弓形虫感染可导致宿主细胞的miRNAs差异表达,差异表达的miRNAs可能在弓形虫和宿主细胞的相互作用中起调控作用。Objective： To investigate microRNAs differential expression of human foreskin fibroblasts （HFFs） during Toxoplasma gondii infection. Methods： The microRNAs differential expression of HFFs during Toxoplasma gondii infection was analyzed by microarray, and further validated by quantitative realtime RT-PCR and Northern blot. Results： The result demonstrated that the level of 46 mature miRNAs upregulated more than 2-fold and 37 mature miRNAs down-regulated more than 2-fold in Toxoplasma gondii infected HFFs, and the validate result of 4 miRNAs level by quantitative realtime RT-PCR and Northern blot was consistent with the microarray data. Conclusion： Toxoplasma gondii infection modulates host miRNAs expression, and differential expressed miRNAs may play an important role on the interaction of the host- Toxoplasma gondii.

关 键 词：弓形虫 人包皮成纤维细胞 微小RNA 基因芯片 

分 类 号：R382.5[医药卫生—医学寄生虫学]