氨基酸胶囊对舰艇官兵Th1／Th2细胞因子和可诱导共刺激分子CD28表达的影响  

Effects of amino acids capsules on the expression of Th1/Th2 cytokines and inducible costimulatory molecules CD28 in the naval ship crew

作　　者：李东涛[1] 刘保海[1] 徐纪平[1] 孙金山[1] 黄文[1] 李兆申[1] 

机构地区：[1]第二军医大学附属长海医院野战内科学研究所,上海200433

出　　处：《中华航海医学与高气压医学杂志》2013年第3期152-154,168,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：军队“十一五”医药卫生科研基金重点课题（082022）;广东省深圳市科技局2009年重点课题

摘　　要：目的探讨氨基酸胶囊对舰艇官兵Th1／Th2细胞因子和可诱导共刺激分子CD28的影响。方法采用数字表法将100名舰艇官兵随机分为氨基酸胶囊组和安慰剂组（对照组），分别给予氨基酸胶囊和安慰剂胶囊，在气温28～35℃，湿度72％-81％环境和正常参加军事作业情况下，连续服用14d，分别在第1、15天用ELISA法检测官兵血清γ-干扰素（IFN-γ）和白细胞介素-4（IL-4）表达水平，流式细胞术检测外周血单个核细胞CD28表达指标CD4＋、CD8＋、CD28＋、CD＋ CD28＋、CD8＋CD28＋。结果实验第15天，胶囊组官兵IFN-γ和IFN-γ／IL-4的值分别为（193．51±19．63）ng／L和（102．86±20．5）明显高于对照组（176．04±18．26）ng／L和（72．55±27．18），差异有统计学意义（P〈0．05），而IL-4、共刺激分子CD4＋、CD28＋、CD4＋CD28＋、CD8＋CD28＋均明显低于对照组（P〈0．05），差异有统计学意义。结论氨基酸胶囊能通过调节可诱导共刺激分子的表达水平，纠正体内的Th2偏移和Th1／Th2失衡，从而提高舰艇官兵机体免疫力。Objective To investigate the effects of amino acids capsules on the expression of Th1/Th2 cytokines and inducible costimulatory molecules CD28 in the naval ship crew. Methods One hundred naval personnel were randomly divided into 2 groups： the amino acids capsule group and the placebo group. The 2 groups were respectively given amino acids capsules and placebo capsules for 14 days at air temperature of 28 - 35 ℃ and humidity of 72% - 81%. Expressions of serum T interferon （IFN-γ） and interferon-4 （ IL-4 ） were detected with ELISA at day 1 and day 15 respectively. In the mean time, the expression levels of CD4＋ , CD8 ＋ , CD28 ＋, CD4 ＋ CD28 ＋ and CD8 ＋ CD28 ＋ in the peripheral blood were detected with flow cytometry （FCM）. Results At day 15, the levels of IFN-T and IFN-γ/IL-4 for the capsule group were （ 193.51 ±19.63） ng/L and （ 102.86 ±20.50）ng/L, which were significantly higher than those of the placebo group （176.04 ±18.26 ）ng/L and （72.55 ± 27.18 ）ng/L, with significantly statistical difference （P 〈 0.05 ）, while the levels of IL-4, CD4 ＋, CD28 ＋, CD4 ＋ CD28 ＋ and CD8 ＋ CD28 ＋ fr the capsule group were significantly lower than those of the placebo group （ P 〈 0.05 ）, also with statistical significance. Conclusions Amino acids capsules could regulate the expression levels of ICOS and correct the Th2 type immune polarization and Th1/Th2 imbalance, and as a result, it could enhance the immunological function of the naval ship crew.

关 键 词：氨基酸 TH1 Th2平衡 可诱导共刺激分子 CD28 

分 类 号：R83[医药卫生—航空、航天与航海医学]