HO-1在银屑病患者皮损及外周血单一核细胞中的表达  

Expression of HO-1 in skin lesions and peripheral blood mononuclear cell of the patients with psoriasis vulgaris

作　　者：马良娟[1] 宋颖[2] 姚军英[1] 柏冰雪[1] 

机构地区：[1]哈尔滨医科大学附属第二医院皮肤科,150081 [2]黑龙江省医院皮肤科,150036

出　　处：《中国麻风皮肤病杂志》2013年第7期440-443,共4页China Journal of Leprosy and Skin Diseases

基　　金：黑龙江省教育厅科学技术研究项目(编号:12511186)

摘　　要：目的:检测寻常型银屑病(PV)患者皮损及外周血单一核细胞(PBMC)中血红素氧合酶-1(HO-1)mRNA和蛋白的表达。方法:逆转录-聚合酶链反应(RT-PCR)法和免疫组化方法检测20例PV患者和10例正常人皮肤及PBMC中HO-1 mRNA和蛋白的表达。结果:PV患者及正常对照组皮肤中HO-1mRNA表达相对含量分别为1.32±0.29和0.46±0.10,HO-1的免疫组化评分分别为10.25±2.36和2.65±1.03:患者及对照组PBMC中HO-1mRNA相对含量分别为0.25±0.14和0.22±0.16,HO-1染色阳性细胞的百分比为11.7%±3.44%和10.5%±4.06%,PV皮损中HO-1的mRNA和蛋白表达明显高于正常对照组(P<0.05),而PV患者外周血PBMC中HO-1 mRNA和蛋白的表达水平与正常对照组差异无显著性(P>0.05)。结论:HO-1可能参与了银屑病皮损的发病过程。Objective：To detect the expression level of heme oxygenase-1 （HO-1 ） mRNA and protein in skin lesions and peripheral blood mononuclear cell （PBMC） of the patients with psoriasis vulgaris （PV）. Methods： RT-PCR and immunohistochemical staining were applied to determine the expression of HO-1mRNA and protein in skin lesions and PBMC from 20 PV patients and 10 normal controls. Results： The levels of HO-1 mRNA in skin lesions of patients with PV and normal controls were （1.32±0.29） and （0.46± 0.10） respectively, the immunohistochemical score of HO- 1 protein were （10.25±2.36） and （ 2.65± 1.03） respectively. The levels of HO- 1 mRNA in PBMC of patients and normal controls were （0.25±0.14） and （0.22 ±0.16） respectively. The positivity of immunohistochemical staining of HO-1 protein in the patients and con- trois were （11.7±3.44）% and （10.5±4.06）% respectively. The content of HO-lmRNA and the expression level of HO- 1 protein in skin lesions of patients with PV was obviously higher than those in controls （ P〈 0.05）. However, there was no significant difference in the content of HO-1 mRNA and the expression level of HO-1 protein in PBMC between the two groups （P〉0.05）. Conclusion： HO-1 may be involved in the pathogenesis of PV.

关 键 词：寻常型银屑病 血红素氧合酶-1 免疫组化 逆转录一聚合酶链反应 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]