GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis  

作　　者：Li-li SUN Zhong-jing ZHOU Li-jun AN Yan AN Yong-qin ZHAO Xiao-fang MENG Clare STEELE-KING Yin-bo GAN 

机构地区：[1]Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University [2]Centre for Novel Agricultural Products, Department of Biology, University of York

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第7期563-569,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Natural Science Foundation of China (Nos. 30970167 and 31228002);the Zhejiang Qianjiang Talent Program (No. 2010R10084);the Zhejiang Provincial Natural Science Foundation of China (No. Z31100041);the Zhejiang Province Foundation for Returned Scholars (No. 20100129), China

摘　　要：Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor- phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of en doreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS （GIS） is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endore- duplication regulator SIAMESE （SIM）. Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantty reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.

关 键 词：Arabidopsis thaliana GLABROUS INFLORESCENCE STEMS (GIS) ENDOREDUPLICATION SIAMESE (SIM) Trichome branching Genetic interaction 

分 类 号：Q943.2[生物学—植物学]