电针对VD大鼠海马神经细胞PKCmRNA、mGluRs和AMPAR表达的影响  

Effects of electroacupuncture on expression of PKC mRNA,mGIuRs and AMPAR in hippocampus of rats with vascular dementia

作　　者：韦登明[1] 蒋雯雯[1] 贾丛林 胡荫[1] 王欣[1] 赵晶[1] 赵鑫[1] 王琳[1] 王钦文[1] 

机构地区：[1]宁波大学医学院病理教研室,宁波315211 [2]奉化市人民医院

出　　处：《中华物理医学与康复杂志》2013年第7期527-531,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：浙江省自然科学基金项目（Y2080445）;宁波市科技局自然科学基金项目（2009A610161）;宁波大学王宽诚幸福基金资助

摘　　要：目的观察电针对血管性痴呆（VD）大鼠海马神经细胞PKC mRNA、mGluRs、AMPAR表达的影响，探讨电针的治疗作用机制。 方法将SD大鼠随机分为假手术组、模型组和电针组，每组10只。模型组和电针组采用重复脑缺血再灌注方法建立VD大鼠模型。将电针组大鼠放入大鼠固定器中，暴露大鼠头部和背部，将电针浅刺入大鼠“百会”、“大椎”穴,每天电针1次，留针20 min，连续治疗10 d。3组大鼠均于造模10 d后采用逆转录聚合酶链式反应（RT-PCR）检测海马神经细胞PKC mRNA，免疫组织化学染色技术观察脑组织海马神经细胞mGluRs、AMPAR染色结果。 结果模型组海马PKC mRNA表达较假手术组降低，而与模型组比较，电针组海马PKC mRNA表达显著增加，差异有统计学意义（P〈0.05）。海马mGluRs免疫阳性细胞积分光密度在假手术组、模型组和电针组分别为（58.6±3.6）、（36.3±2.5）和（51.5±4.8），与假手术组比较，模型组海马mGluRs免疫阳性细胞积分光密度显著降低，差异有统计学意义（P〈0.01）；而与模型组比较，电针组海马mGluRs免疫阳性细胞积分光密度显著增加，差异有统计学意义（P〈0.05）；海马AMPAR免疫阳性细胞积分光密度在假手术组、模型组和电针组分别为（66.5±2.8）、（40.1±5.1）和（58.3±4.6），与假手术组比较，模型组海马AMPAR免疫阳性细胞积分光密度显著降低，差异有统计学意义（P〈0.01），而与模型组比较，电针组海马AMPAR免疫阳性细胞积分光密度显著增加，差异有统计学意义（P〈0.05）。 结论电针可增加VD大鼠海马PKC mRNA、mGluRs和AMPAR表达。电针大鼠“百会”、“大椎”穴改善大鼠学习记忆能力的机制可能与提高海马mGluRs、AMPAR和PKC mRNA表达有关。Objective To observe the effect of electroacupuncture on the hippocampal PKC mRNA, mGluRs and AMPAR expression in rats with vascular dementia （VD） , so as to explore the underlying mechanisms of electroacupuneture （EA） therapy for VD. Methods Thirty Sprague-Dawley rats were randomly divided into a sham-operation group, a model group and an EA group（ 10 in each group）. VD model was established by repeated isehemia/reperfusion. Then the rats were placed in the rat fixer with their heads and back exposed. The electro-acupuncture were superficially inserted into ＂ Baihui＂ （GV20） and ＂ Dazhui＂ （DU14） in rats. EA was applied to ＂Baihui＂ （GV20）, ＂Dazhui＂ （DU14） in EA group for 20 rain, once daily for 10 days. The PKC mRNA, mGluRs and AMPAR expression in hippocampal tissue were examined by RT-PCR and immunohistochemieal assay. Results The expression level of PKC mRNA in hippoeampus of the model group was lower than that of the sham- operation group. Compared with the model group, the level of PKC mRNA was significantly increased in the EA group （P 〈 0.05 ）. The mGluRs immunoreactive cell integrated optical density （IOD） in hippocampus in the sham-operation group, the model group and the EA group were （58.6 ±3.6）, （36.3 ±2.5） and （51.5 ±4.8）, respectively; the mGluRs immunoreactive ceil IOD in hippocampus in the model group was reduced significantly （ P 〈 0.01 ） when compared with the sham-operation group, The mGluRs immunoreaetive cell IOD in hippocampus in the EA group was increased significantly as compared with the model group （ P 〈 0.05）. AMPAR immunoreactive cell IOD in hippocampus in the sham-operation, the model group and the EA group were （66. 5 ± 2. 8） ,（40.1 ± 5.1 ） and （58.3 ± 4.6） , respectively, and compared with the sham-operation group, the AMPAR immunoreactive cell IOD in hippocampus in the model group was reduced significantly. The difference of the AMPAR immunoreactive cell IOD in hippocampus between the sham

关 键 词：血管性痴呆 电针 学习记忆能力 PKC mRNA MGLURS AMPAR 

分 类 号：R285.5[医药卫生—中药学]