当归四逆汤成分组合预处理对心肌缺血再灌注损伤大鼠心肌微血管内皮细胞凋亡的影响  

Effect of combination of Dangguisini decotion components pretreatment on microvessel endothelial cell apoptosis in MIRI rats

作　　者：钱国强[1] 尹晓峰[1] 高宇勤[2] 蔡川[3] 郭军[3] 彭霞[3] 赵国平[3] 

机构地区：[1]黄淮学院护理学系,河南驻马店463000 [2]十堰市人民医院心内科 [3]暨南大学医学院

出　　处：《中国老年学杂志》2013年第14期3350-3353,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助(No.81173189);河南省科技攻关项目(No.122102310473);黄淮学院青年骨干教师资助计划

摘　　要：目的观察当归四逆汤成分组合对心肌缺血再灌注损伤(MIRI)大鼠心肌微血管内皮细胞(MMVEC)凋亡的影响。方法将32只大鼠分为sham组,I/R组,PPC组,PPC+L组,建立大鼠MIRI模型,电镜观察大鼠MMVEC凋亡及血清NO。结果与sham组比较,I/R组MMVEC凋亡率明显升高(P<0.05);与I/R组比较,PPC组MMVEC凋亡率明显下降(P<0.05);与PPC组比较,PPC+L组MMVEC凋亡率有所升高(P<0.05),凋亡率与该组大鼠血清NO含量成反比。结论当归四逆汤成分组合可有效抑制大鼠MMVEC凋亡,其机制与调节NO的生成有关。Objective To investigate the protective effect of combination of Dangguisini decotion components（GFPC） on microvessel endothelial cell（MMVEC） apoptosis in MIRI SD rats.Methods 32 SD rats were divided into sham（non-ischemic conditions）,I/R（heart subject to ischemia-reperfusion）,PPC（heart subjected to ischemia-reperfusion treated with GFPC）,PPC＋L groups（heart subjected to ischemia-reperfusion treated with GFPC and pre-treated with the eNOS inhibitor,Nω-Nitro-L-arginine methylester,L-NAME,15min before reperfusion,30 mg/kg）,8 per group.After the model,the left ventricular apical section was fixed and then sliced,morphological changes of MMVEC were observed by electron microscopy,apoptosis index（AI） of MMVEC was observed by means of TUNEL extracting blood from abdominal aortic,NO was tasted by methods of nitrate reduction.Results Compared with sham group,MMVEC apoptosis rate of I/R group was significantly higher（P0.05）.Compared with I/R group,MMVEC apoptosis rate of PPC group was decreased（P0.05）.Compared with PPC group,MMVEC apoptosis rate of PPC ＋ L group was increased（P0.05）.Conclusions GFPC could decrease apoptosis rate of MMVEC,which mechanism is by regulating the production of NO.

关 键 词：当归四逆汤 心肌缺血再灌注损伤 心肌微血管内皮细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]