槲皮素对肉鸡脂肪细胞环腺苷酸信号通路的作用  

Effect of Quercetin on cAMP Signaling Pathway in Chicken Adipocytes

作　　者：欧阳文文[1] 李垚[1] 赵伟[1] 王明昊[1] 金芳[1] 

机构地区：[1]东北农业大学动物营养研究所,哈尔滨150030

出　　处：《中国农业科学》2013年第13期2769-2776,共8页Scientia Agricultura Sinica

基　　金：国家科技支撑计划项目(2006BAD14B02-8-1);哈尔滨市科学技术局科技创新人才研究专项资金(2011RFLXN015)

摘　　要：【目的】研究槲皮素对肉鸡脂肪细胞甘油三酯沉积过程中环腺苷酸(cyclic adenosine monophosphate,cAMP)信号通路的作用。【方法】将脂肪细胞随机分5组,每组6个重复;空白对照组为基础培养液,溶剂对照组为含2‰二甲基亚砜(DMSO)的培养液,试验组为含10、20和40 mg.L-1槲皮素的培养液,分别于24、48和72 h收集细胞及培养液;ELISA法测定乙酰CoA羧化酶(acetyl CoA carboxylase,ACC)、脂肪酸合成酶(fatty acidsynthase,FAS)、脂蛋白脂酶(lipoprotein lipase,LPL)、cAMP、腺苷酸环化酶(adenylate cyclase,AC)、磷酸二脂酶(phosphodiesterase,PDE)、蛋白激酶(protein kinase A,PKA)含量;紫外分光光度计测定甘油三酯(triacylglycerides,TG)含量。【结果】与空白对照组相比,①溶剂对照组各指标均无显著差异(P>0.05);②20和40 mg.L-1槲皮素组ACC、FAS、LPL、TG和PDE含量显著降低(P<0.05),cAMP和PKA含量显著增加(P<0.05);③3个槲皮素组AC含量均无显著差异(P>0.05)。【结论】一定剂量槲皮素可通过调控cAMP信号通路抑制脂肪合成,并减少脂肪沉积。[Objective] This experiment was conducted to study the effects of quercetin on cAMP signaling pathway in broilers adipocytes. [Method] Adipocytes were randomly allocated into 5 groups with 6 replicates per group. Adipocytes were cultured in basal medium, 2%0 DMSO medium or addition of 10, 20, 40 mg＇Ll quercetin medium for 24 h, 48 h and 72 h, respectively. Then adipocytes and medium were collected for measuring the contents of ACC, FAS, LPL, cAMP, AC, PDE, PKA by ELISA and TG by ultraviolet spectrophotometer, respectively. [Result] Compared with the control, all of these indicators of adipocytes were not significantly affected by 2%0 DMSO （P〉O.05）. The contents of ACC, FAS, LPL,TG~ and PDE were significantly decreased in medium supplemented with 20 mg＇Ll and 40 mg-L1 quercetin （P〈0.05）, cAMP and PKA contents were significantly increased （P 〈0.05）; Intracellular AC content was not significantly affected by quercetin （P〉0.05）. [Conclusion] The results indicated that quercetin inhibited lipogenesis and fat deposition via regulating cAMP signaling pathway.

关 键 词：槲皮素 脂肪细胞 脂肪代谢 环腺苷酸 信号通路 

分 类 号：S831.5[农业科学—畜牧学]