两种中药成分对肉鸡脂质代谢的影响  

Effects of Two Chinese Medicinal Ingredients on Lipid Metabolism in Broilers

作　　者：张向杰[1] 孙耀贵[1] 程佳[1] 李宏全[1] 王俊东[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国农业科学》2013年第13期2788-2795,共8页Scientia Agricultura Sinica

基　　金：山西省自然科学基金(20031070);山西省青年科技研究基金(2011021007-4)

摘　　要：【目的】探讨日粮中分别添加山楂叶总黄酮、何首乌二苯乙烯苷及二者同时添加对肉鸡屠宰性能、血清生化指标及脂质代谢相关功能基因表达的影响。【方法】选用1日龄AA肉鸡128只(雄),随机分为4个处理组,即空白对照组(饲喂玉米-豆粕型基础日粮)、Ⅰ组(在日粮中添加300 mg.kg-1山楂叶总黄酮)、Ⅱ组(在日粮中添加150 mg.kg-1何首乌二苯乙烯苷)和Ⅲ组(在日粮中添加300 mg.kg-1山楂叶总黄酮和150 mg.kg-1何首乌二苯乙烯苷)。每组4个重复,每个重复8只。【结果】①与对照组相比,Ⅰ组的全净膛率、血清高密度脂蛋白胆固醇、肝脏组织中过氧化物酶增生物激活受体α和肉毒碱棕榈酰基转移酶-Ⅰ的表达量均显著升高(P<0.05);腹脂率、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、肝脏组织中固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶的表达量均显著降低(P<0.05)。②与对照组相比,Ⅱ组的屠宰率、血清高密度脂蛋白胆固醇、游离脂肪酸、肝脏组织中过氧化物酶增生物激活受体α和肉毒碱棕榈酰基转移酶-Ⅰ的表达量均显著升高(P<0.05);腹脂率、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、肝脏组织中固醇调节元伴结合蛋白-1、乙酰辅酶A羧化酶、脂肪酸合成酶的表达量均显著降低(P<0.05)。③Ⅲ组的屠宰率、半净膛率、全净膛率、胸肌率、腿肌率和皮下脂肪厚均显著高于对照组(P<0.05);屠宰率、全净膛率显著高于Ⅰ组、Ⅱ组(P<0.05);腿肌率显著高于Ⅰ组(P<0.05);腹脂率显著低于对照组、Ⅰ组、Ⅱ组(P<0.05)。血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇均显著低于对照组、Ⅰ组、Ⅱ组(P<0.05);血清高密度脂蛋白胆固醇、游离脂肪酸、总蛋白、白蛋白均显著高于对照组(P<0.05);高密度脂蛋白胆固醇、游离脂肪酸显著高于Ⅰ组、Ⅱ组(P<0.05);肝脏组织中的过�[ Objective ] The experiment was conducted to investigate the effects of TFHL, TSG and TFHL＋TSG supplement in daily ration for broilers on slaughter performance, serum biochemical index and expressions of lipid metabolism related genes. [Method] One hundred and twenty-eight male broilers （1 day old） were divided randomly into 4 groups：control group （fed a basal com-soybean meal diet）, group I （basal diet＋ 300 mg＇kgl TFHL）, group II （basal diet＋ 150 mg＇kgl TSG） and group III（basal diet ＋300 mg＇kgl TFHL＋ 150 mg＇kg-I TSG）. Four replicates were designed for each group and each replicate had 8 broilers. [Result] Compared with the control group, the eviscerate percentage, serum HDL-C, mRNA expressions of PPARct, CPT- I in liver tissue in group I were significantly higher （P〈0.05）; the abdominal fat percentage, serum TG~ TC and LDL-C, mRNA expressions of SREBP-1, ACC and FAS in liver tissue were significantly decreased （P〈0.05）. Compared with the control group, the dressing percentage, serum HDL-C and FFA, mRNA expressions of PPARc~, CPT- I in liver tissue in group II were significantly higher （P〈 0.05）. The abdominal fat percentage, serum TG~ TC and LDL-C, mRNA expressions of SREBP-1, ACC and FAS in liver tissue were significantly decreased （P〈0.05）. Compared with the control group, the dressing percentage, semi-eviscerate percentage, eviscerate percentage, breast muscle percentage, leg muscle percentage and subcutaneous fat thickness in group III were significantly higher （P 〈0.05）. The dressing percentage and eviscerate percentage in group III were much higher than those in groups I and II （P〈0.05）, and the leg muscle percentage was much higher than group I （P〈0.05）. The abdominal fat percentage in group III was significantly lower than that of control group, groups I and II （P〈0.05）. The TG, TC and LDL-C of serum in group III were significantly lower than those of the control group, groups I and II （P〈0.05）.Compared with t

关 键 词：山楂叶总黄酮 何首乌二苯乙烯苷 肉鸡 屠宰性能 生化指标 脂质代谢 

分 类 号：S831[农业科学—畜牧学]