雄激素缺乏对雄性大鼠肝脏、肌组织及胰岛病理改变的影响  

Influence of testosterone deficiency on pathological changes of liver, skeletal muscle and pancreatic islet of male rats

作　　者：许潇[1] 夏芳珍[1] 翟华玲[1] 吴晖[1] 张惠新[1] 陆颖理[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院内分泌代谢科,上海200011

出　　处：《上海交通大学学报（医学版）》2013年第7期955-959,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(09140903700)~~

摘　　要：目的观察雄激素缺乏对雄性大鼠肝脏、肌组织及胰腺病理结构的影响。方法健康SD雄性大鼠随机分为正常对照组(n=5,给予假去势)、去势组(n=7,施行去势手术,建立雄激素缺乏模型)和替代组(n=5,施行去势手术,每日腹腔注射丙酸睾酮12.5 mg/kg替代治疗,连续10周),30周后取各组大鼠血清,放射免疫法测定血清睾酮水平;取各组大鼠肝脏、骨骼肌及胰腺组织,HE染色后行光学显微镜观察。结果与正常对照组和替代组比较,去势组血清睾酮水平显著降低(P<0.01)。光学显微镜观察示去势组大鼠肝细胞胞质内见大小不等的脂肪空泡,胞核缩小;替代组大鼠肝脏空泡数量较去势组减少。去势组大鼠肌组织大部分横纹消失,肌纤维结构紊乱,可见椭圆形透亮脂肪滴;替代组大鼠肌组织结构接近于正常对照组。正常对照组胰岛β细胞形态正常,去势组和替代组大鼠胰岛β细胞接近于正常对照组。结论脂质沉积对去势后雄性大鼠肝脏、肌组织造成病理损伤,外源性睾酮替代治疗可以一定程度上改善病变。Objective To observe the influence of testosterone deficiency on pathological changes of liver, skeletal muscle and pancreatic islet of male rats. Methods Healthy male SD rats were randomly assigned into normal control group （n = 5, sham castration was performed）, castrated group （n = 7, testosterone deficiency model was established with castration） and replacement group （n = 5, castration was performed, and intraperitoneal injection of 12.5 mg/kg testosterone propionate was conducted for 10 weeks）. Thirty weeks later, serum concentration of testosterone was measured by radioimmunoassay in each group. The tissues of liver, skeletal muscle and pancreatic islet were obtained, and were observed by light microscopy with HE staining. Results The serum concentration of testosterone in castrated group was significantly lower than those in normal control group and replacement group （P 〈 0.01）. Light microscopy revealed that there was accumulation of lipid droplets in the cytoplasm of liver in castrated group, and there were less lipid droplets in replacement group than in castrated group. The size of nuclei in castrated group was smaller than that in normal control group. In castrated group, most skeletal muscle tissues were not clear in cross-sectional diameter, the pattern of cross striation in muscles disappeared, swelled and arranged in disorder, and the round lipid droplets were seen focally. However, the structure of muscle tissues in replacement group was similar with that in normal control group. The histological structure of pancreatic islet [β cells was normal in normal eontrol group, and the histological structure in castrated group and replacement group was similar with that in normal control group. Conclusion Fat deposition may cause pathological damage to liverand muscle tissues of male rats with castration, and exogenous testosterone replacement therapy may improve the pathological changes to some degree.

关 键 词：雄激素缺乏 肥胖 脂质 

分 类 号：R96[医药卫生—药理学]