结核分枝杆菌Rv0315重组蛋白ELISPOT辅助诊断活动性肺结核的应用价值  

Diagnostic value of ELISPOT by recombinant Rv0315 protein of Mycobacterium tuberculosis in detection of active pulmonary tuberculosis

作　　者：周业成[1] 陈翠翠[2] 叶娟[3] 赵俊伟[4] 玄松花[3] 杨志荣[1] 张舒林[4] 

机构地区：[1]四川大学生命科学学院,成都610065 [2]郑州大学第一附属医院,郑州450001 [3]河南中医学院分子生物学教研室,郑州450008 [4]上海交通大学基础医学院病原生物学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2013年第7期970-974,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委科技支撑项目(12441903300);国家自然基金(81271794);"十二五"国家科技重大专项(2013ZX10003002-005)~~

摘　　要：目的用酶联免疫斑点试验(ELISPOT)评价结核分枝杆菌重组蛋白Rv0315在活动性肺结核病诊断中的应用价值。方法选取初治肺结核病患者25例和健康体检者28名分别作为结核组和对照组,应用Rv0315蛋白作为抗原对受试者外周血单核细胞进行ELISPOT检测斑点形成细胞(SFCs)的数量,判断结核分枝杆菌感染情况,并与结核早期分泌靶向抗原(ESAT6)和培养滤液蛋白10(CFP10)抗原的检测结果进行比较。结果以Rv0315、EAST6和CFP10作为抗原,用ELISPOT方法检测结核分枝杆菌感染的敏感度分别为84.0%(21/25)、80.0%(20/25)和92.0%(23/25),特异度分别为89.2%(25/28)、89.2%(25/28)和92.8%(26/28),其中Rv0315的敏感度高于EAST6但低于CFP10。结论应用重组蛋白Rv0315作为抗原建立的ELISPOT方法具有应用于活动性肺结核病辅助诊断的潜在价值。To investigate the application of enzyme-linked immunospot assay （ELISPOT） by recombinant Rv0315 protein of Mycobacterium tuberculosis in the auxiliary diagnosis of active pulmonary tuberculosis. Methods A total of 25 patients with initial pulmonary tuberculosis （ tuberculosis group） and 28 healthy people （ control group） were enrolled. The peripheral blood mononuclear cells of all participants were co-cultured with Rv0315, earlier secreted antigen target 6 （EAST6） and culture filtrate protein 10 （CFPIO） antigens, and spot forming cells （SFCs） were enumerated by ELISPOT. Results The values of sensitivity of Rv0315, EAST6 and CFP10 antigens by ELISPOT were 84.0% （21/25）, 80.0% （20/25） and 92.0% （23/25） respectively, and those of specificity were 89.2% （25/28）, 89.2% （25/28） and 92.8% （26/28） respectively. Rv0315 was more sensitive than EAST6, but was less sensitive in comparison with CFP10 in diagnosis of Mycobacterium tuberculosis infection. Conclusion ELISPOT with recombinant Rv0315 protein as antigen has the potential value in the auxiliary diagnosis of active pulmonary tuberculosis.

关 键 词：结核分枝杆菌 Rv0315 酶联免疫斑点技术 细胞免疫 

分 类 号：R521[医药卫生—内科学]