基于谷胱甘肽-壳聚糖自组装的葡萄糖生物传感器  

Glucose Biosensor Based on the Chitosan/Glutathione Composite Film

作　　者：高先娟[1] 王怀生[2] 蔡青云[3] 

机构地区：[1]山东万杰医学院、山东省生物医学工程技术重点实验室,山东淄博255213 [2]聊城大学化学化工学院,山东聊城252000 [3]湖南大学化学化工学院、化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《分析测试学报》2013年第7期872-876,共5页Journal of Instrumental Analysis

基　　金：山东省教育厅高校科研发展计划项目(J13LD59)

摘　　要：采用壳聚糖-谷胱甘肽复合膜固定葡萄糖氧化酶构建电流型葡萄糖生物传感器。通过循环伏安法对酶膜状态进行表征,实验结果表明,壳聚糖-谷胱甘肽复合膜可以辅助电子传递,提高电极的电流响应。选用正交表L9(34)设计实验方案,分析最佳实验条件。在优化条件下,该传感器对葡萄糖溶液浓度有良好的线性关系,线性范围为1～18 mmol/L,检出限为1.3 mmol/L。实验表明,此传感器具有响应快、稳定性及选择性良好的特点。适用于临床尿样中葡萄糖的测定。A chitosan/glutathione composite film combined with glucose oxidase was employed to fabricate an amperometric glucose biosensor.The enzyme electrode was characterized by cyclic voltammetry.The experimental results showed that,chitosan and glutathione composite film can assist in electron transfer,improve response electrode current.Using orthogonal table L9（34） experiment design,the best experimental conditions were obtained by visual analysis.Under the optimized conditions,the sensor has a good linear relationship with concentration of glucose solution in the range of 1-18 mmol/L,the detection limit is 1.3 mmol/L.Experiments showed that,this sensor has the advantages of fast response,good stability and selectivity,and is suitable for the determination of glucose in urine sample.

关 键 词：金电极 壳聚糖 葡萄糖氧化酶 生物传感器 谷胱甘肽 正交试验 

分 类 号：O657.1[理学—分析化学] TS236.3[理学—化学]