蝴蝶兰丛生芽快繁体系的建立  

Mass propagation of clustered shoots of Phalaenopsis

作　　者：谭鹏鹏[1] 彭方仁[1] 江荣翠[2] 尚杨娟 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]南京邮电大学 [3]合肥大蜀山森林公园管理处

出　　处：《林业科技开发》2013年第4期80-84,共5页China Forestry Science and Technology

基　　金：国家"十二五"科技支撑计划课题子项目(编号:2012BAD21B00)

摘　　要：以蝴蝶兰花梗腋芽为外植体,对消毒方法、培养基种类、激素浓度、褐化控制等因素进行研究,结果表明:花梗切为2～3 cm带腋芽段,预处理后用0.1%升汞灭菌15 min,灭菌成功率达90%;花梗腋芽最适诱导培养基为MS或1/2MS+6-BA 3.0 mg/L;丛生芽诱导最适培养基为1/2MS+6-BA 7.5 mg/L+NAA 1.0 mg/L;生根最适培养基为1/2MS+NAA 1.0 mg/L+AC 1.0 g/L+香蕉泥60 mg/L。建立了一套通过诱导丛生芽进行蝴蝶兰快繁的有效途径。With an axillary bud of flower stalks as explants, sterilization methods, medium types, hormone concentration and browning control were studied. The results showed that the 2-3 cm cutting segments with an axillary bud of flower stalks were soaked into 0.1% HgC12 for 15 min after series of pre-treatment. The aseptic rate could reach 90%. The suitable medium for primary culture was MS or 1/2MS ＋6-BA 3.0 mg/L. The suitable medium for subculture was 1/2MS ＋6-BA 7.5 mg/L ＋ NAA 1.0 mg/L. The suitable medium for rooting was 1/2MS ＋ NAA 1.0 mg/L ＋ AC 1.0 g/L ＋ banana pulp 60 mg/L. A method for mass propagation was found through inducing clustered shoots.

关 键 词：蝴蝶兰 花梗腋芽 激素浓度 丛生芽 

分 类 号：S791.28[农业科学—林木遗传育种]