脑脊液白细胞显微镜计数法的改良  

Improvement of the current routine method of WBC counting in cerebrospinal fluid

作　　者：黄丽春[1] 岑雨贞[1] 郑磊[1] 熊铁[1] 陈雅妮[1] 孙德华[1] 

机构地区：[1]南方医科大学南方医院检验科,广州510515

出　　处：《重庆医学》2013年第21期2503-2504,2507,共3页Chongqing medicine

摘　　要：目的对现用脑脊液白细胞显微镜计数法进行改良,寻求最佳冰醋酸浓度及与脑脊液混合的最适比例,提高脑脊液白细胞计数的准确度、精密度。方法将不同浓度冰醋酸与脑脊液标本进行不同比例混合,形成28种组合方法。标本稀释后5min内对28种组合方法进行显微镜白细胞计数,同时以现用方法进行计数,每种方法均计数2次取均值。计数20例脑脊液标本后比较每种方法所得的白细胞计数均值与变异系数。结果脑脊液与5%冰醋酸的混合比例为60∶40时,脑脊液白细胞计数结果最高(189个/μL),明显高于现用方法(161个/μL);变异系数也最小(7%),明显低于现用方法(18%)。结论使用5%的冰醋酸、且脑脊液与其混合比例为60∶40时,白细胞计数结果的准确性、精密度达到最佳。Objective To improve the reliability and accuracy of WBC counting in cerebrospinal fluid （CSF）, this article is stud- ying the improved method of WBC counting in CSF by finding out the optimum percentage of CSF specimen with the most suitable concentration of acetic acid. Methods CSF specimen was mixed with different acetic acid at different ratio respectively. WBC counts were performed in 5 minutes on diluted samples of various concentrations. A series of 20 CSF specimens were analyzed via the proposed assay and conventional method. The average value and coefficient of variation （CV） of WBC count of each sample were c compared and analyzed. Results The optimum percentage of CSF sample was obtained at 60 ~ 40 ratio . In this percentage, the maximal WBC count （189%） was obtained compared that of conventional method （161%）. Moreover, the CV of the WBC counts in this percentage （70% ） was also lower than that of the conventional method （18 %）. Conclusion The reliability and accur- ancy of WBC counting in CSF was the optimum percentage of CSF specimen and 5% acetic acid was 60：40. It may lead to a more reliable, accurate and standard way of WBC counts in CSF.

关 键 词：脑脊液 白细胞计数 现用显微镜计数法 改良显微镜计数方法 稀释冰醋酸 

分 类 号：R446.1[医药卫生—诊断学]