抗精子抗体与精子形态的相关性调查  

Correlation investigation of anti-sperm antibodies and sperm morphology

作　　者：杨大干[1] 吕俊霞[1] 姚冬英[1] 沈乃仙[1] 

机构地区：[1]浙江大学医学院附属第一医院检验科,浙江杭州310003

出　　处：《检验医学》2013年第7期590-594,共5页Laboratory Medicine

基　　金：浙江省人口和计划生育委员会科技项目(2010-KY-1-28)

摘　　要：目的调查抗精子抗体(AsAb)对精子形态的影响。方法选取AsAb阳性组51例、疾病对照组40例和健康对照组47名,采用Diff-Quik染色法按世界卫生组织(WHO)精子形态分类标准进行精子形态评估,计算畸形精子指数(TZI)和精子畸形指数(SDI)。结果 AsAb阳性组的异常形态精子、锥形头、不定形头、颈中段增粗分别为80.2%±7.7%、6.3%±5.5%、11.0%±7.4%、21.9%±9.0%;疾病对照组分别为71.6%±5.8%、4.2%±4.5%、11.1%±4.8%、16.0%±6.9%;健康对照组分别为70.1%±6.4%、3.5%±4.6%、8.4%±4.3%、16.2%±6.9%;上述各指标在3组间差异均有统计学意义(F值分别为28.732、3.867、3.282、4.893,P<0.05或P<0.01)。AsAb阳性组TZI和SDI分别为1.306±0.123、1.057±0.180;疾病对照组为1.255±0.096、0.901±0.119;健康对照组为1.257±0.098、0.882±0.121;3组间差异有统计学意义(F值分别为3.088、19.115,P<0.05或P<0.01)。结论 AsAb阳性可能是异常精子形态增多的原因之一,异常精子形态以锥形头、不定形头、颈中段增粗为主。Objective To investigate the influence of anti-sperm antibodies（AsAb） on sperm morphology.Methods A total of 51 AsAb positive patients,40 control patients and 47 healthy controls were enrolled.The sperm morphology was evaluated according to World Health Organization（WHO） sperm morphology classification standards by Diff-Quik staining method.The abnormal sperm index（TZI） and sperm deformity index（SDI） were calculated.Results In the AsAb positive patients,the percentages of abnormal morphology（%）,cone-shaped head（%）,amorphous head（%） and middle neck thickening sperms（%） were 80.2 ± 7.7,6.3 ± 5.5,11.0 ± 7.4 and 21.9 ± 9.0,respectively.In the control patients,these percentages were 71.6 ± 5.8,4.2 ± 4.5,11.1 ± 4.8 and 16.0 ± 6.9,respectively.In the healthy controls,these percentages were 70.1 ± 6.4,3.5 ± 4.6,8.4 ± 4.3 and 16.2 ± 6.9,respectively.There were statistical significances between the 3 groups as to the above mentioned parameters（F values were 28.732,3.867,3.282 and 4.893,P〈0.05 or P〈0.01）.The TZI and SDI were 1.306 ± 0.123 and 1.057 ± 0.180 in the AsAb positive patients,1.255 ± 0.096 and 0.901 ± 0.119 in the control patients,and 1.257 ± 0.098 and 0.882 ± 0.121 in the healthy controls,respectively.There were statistical significances between the 3 groups（F values were 3.088 and 19.115,P〈0.05 or P〈0.01）.Conclusions The positivity of AsAb is one of the reasons for the increasing of abnormal sperm morphology.Abnormal sperm morphology has mainly cone-shaped head,amorphous head and middle neck thickening sperms.

关 键 词：抗精子抗体 精子形态 多重精子缺陷指数 

分 类 号：R446.11[医药卫生—诊断学]