Analysis of Functional Group of Alkaline Phosphatase from DFRKN-1  

作　　者：Ma Aiying Zhang Kaowen Yang Zhenhua Mou Yi 

机构地区：[1]College of Biological Sciences and Engineering, North University for Nationalities

出　　处：《Plant Diseases and Pests》2013年第1期39-41,44,共4页植物病虫害研究（英文版）

基　　金：Supported by Key Laboratory Project of State Ethnic Affairs Commission (2010SY12)

摘　　要：To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nem-atode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg^2＋ , Ba^2＋ and K^＋ under certain concentrations activated enzyme significantly and Zn^2＋ could inhibit enzyme activ-ity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline pbos-phatase; sulflaydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phospha-tase.To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nem-atode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg^2＋ , Ba^2＋ and K^＋ under certain concentrations activated enzyme significantly and Zn^2＋ could inhibit enzyme activ-ity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline pbos-phatase; sulflaydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phospha-tase.

关 键 词：Destroying fungi of root-knotnematode-1 （ DFRKN-1 ） Alkaline phosphatase Metal ion Organic reagent Functional group 

分 类 号：R341[医药卫生—基础医学]