拟穴青蟹14-3-3ζ基因cDNA的克隆和表达分析  

The full length cDNA cloning and expression of 14-3-3ζ gene in green mud crab (Scylla paramamosain)

作　　者：王晓伟[1] 高洁[1] 张子平[1] 邹志华[1] 贾锡伟[1] 林鹏[1] 王艺磊[1] 

机构地区：[1]集美大学水产学院,农业部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《上海海洋大学学报》2013年第4期496-501,共6页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31072200,41006104);集美大学创新团队基金(2010A001)

摘　　要：利用SMART RACE技术克隆得到拟穴青蟹ERK信号通路的酪氨酸3-加单氧酶/色氨酸5-加单氧酶激活蛋白ζ(14-3-3ζ蛋白)基因。其cDNA全长1 092 bp,编码248个氨基酸。实时定量PCR结果显示14-3-3ζ基因在各组织器官均有表达,但在卵巢中的表达量显著高于其他组织(P<0.05),在卵黄发生中期14-3-3ζ的表达量显著高于增殖期(P<0.05),推测其在青蟹卵巢中发挥重要作用。Green mud crab, Scylla paramamosain, is an important commercial species in aquaculture. Therefore, more and more researches focus on its molecular mechanism of reproduction. ERK pathway is essential for the regulation of gonad development. The 14-3-3 was proved to be bonded to phosphorylated protein and involved in transmission of ERK pathway. The full length cDNA of tyrosine-3-monooxygenase/ tryp-tophane-5- monooxygenase activator protein （14-3-3ζ） from green mud crab were obtained by SMART RACE in this study. The full length cDNA of 14-3-3ζ gene is 1 092 bp encoding a polypeptide of 248 amino acids. The results of real time quantitative PCR revealed that 14-3-3ζ gene was constitutively expressed in examined tissues, and its expression level in ovary was higher than that in the other tissues （ P 〈 0.05 ） , while the expression level of 14-3-3ζ gene in secondary vitellogenesis stage was higher than that in proliferation stage （ P 〈 0. 05 ）. Consequently, we may infer that 14-3-3ζ plays a key role in ovarian development of the crab.

关 键 词：拟穴青蟹 14-3-3ζ基因 ERK信号途径 组织表达 卵巢发育 

分 类 号：S917[农业科学—水产科学]