金黄色葡萄球菌CP5偶联鞭毛蛋白疫苗对小鼠乳腺炎模型的保护作用  

Protective effect of Staphylococcus aureus type 5 capsular polysaccharide conjugated with flagellin vaccine on mouse mastitis model

作　　者：袁林[1] 郭建军[1] 王贤卓[2] 

机构地区：[1]江西省科学院微生物研究所,江西南昌330096 [2]南昌大学生命科学与食品工程学院,江西南昌330031

出　　处：《广东农业科学》2013年第13期120-122,151,F0002,共5页Guangdong Agricultural Sciences

基　　金：江西省科技支撑计划项目(20112BBF60045;20122BBG70093);江西省科学院项目(2010-yyB-02)

摘　　要：以鞭毛蛋白(flagellin)作为载体蛋白,通过化学偶联至金黄色葡萄球菌5型荚膜多糖(CP5),并在小鼠模型上检验多糖疫苗对小鼠存活及其乳腺的保护作用。将小鼠分为4组:生理盐水组、CP5单独免疫组、牛血清白蛋白(BSA)-CP5偶联组和flagellin-CP5偶联组,每组10只,分别进行皮下免疫,均含CP5 10μg;共免疫两次,两次免疫间隔3周。第2次免疫两周后,用金黄色葡萄球菌分别对小鼠腹腔、乳腺进行攻毒,并通过实时定量PCR对乳腺组织细胞因子IL-4、IL-10、IFN-γ、IL-12的表达进行检测。结果表明,CP5偶联鞭毛蛋白疫苗对小鼠乳腺具有显著的保护效果,并在乳腺组织部位诱导了Th1型细胞因子表达,说明鞭毛蛋白是CP5的理想载体蛋白。Taking flagellin as carrier protein, by chemical coupling to Staphylococcus aureus type 5 capsular polysaccharides, we tested the protective effect of polysaccharide vaccine on mouse model for breast. Mice were divided to 4 groups with 10 mice in each, they were immunized with saline, 10 μg CP5 alone, 10 μg CP5 conjugated with BSA （BSA-CP5）, 10 μg CP5 conjugated with flagellin （flagellin-CPS） respectively on the first day and the 21th day. Two weeks after the second immunization, mice were challenged with S.aureus in peritoneal or breast, and cytokines expression of IL-4, IL-10, IFN- γ, IL-12 in breast tissue were quantified by real-time PCR. The results showed that the CP5 conjugated with flagellin vaccine had a protective effect on mice breast, and induced Thl cytokines expression in breast tissue, suggestting that flagellin was an ideal carrier protein for CPS.

关 键 词：5型荚膜多糖 鞭毛蛋白 乳腺 细胞因子 

分 类 号：S857.26[农业科学—临床兽医学]