台盼蓝染色鉴定拟南芥sdl1突变体的细胞死亡  

Identification of Cell Death of Arabidopsis Thaliana Mutant sdl1 by Trypan Blue Staining

作　　者：支添添[1] 周舟[1] 韩成云[1] 任春梅[1,2] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]作物基因工程湖南省重点实验室,长沙410128

出　　处：《作物研究》2013年第3期217-218,223,共3页Crop Research

基　　金：国家自然科学基金项目(30671121)

摘　　要：拟南芥突变体sdl1在光周期为16 h黑暗/8 h光照条件下生长叶片出现先萎蔫后白化现象,采用台盼蓝染色的方法研究萎蔫及白化苗的死亡情况,结果证实突变体sdl1萎蔫处发生细胞死亡,但细胞完全死亡(完全白化)后不能被染色,所以台盼蓝染色只能对突变体sdl1细胞死亡的早期进行鉴定。Phenomenon of leaves wilting and albinism of the Arabidopsis thaliana Mutant sdll seedlings was observed when grew for 5-8 days under 16 hours dark/8 hours light condition. The death status was studied by Trypan blue staining, and the results showed that sdll leaves couldn＇ t be stained when totally whitened, so Trypan blue staining could identify sdll cell death just in the early stage of cell death.

关 键 词：拟南芥 台盼蓝染色 细胞死亡 

分 类 号：Q813.4[生物学—生物工程]