番茄突变体jai1-1高效筛选—再生体系的建立  

Establishment of High Efficient Screening and Regeneration System of Tomato mutant jai1-1 in Vitro

作　　者：范锡麟[1] 王少岭[1] 肖应辉[1] 王志龙[1] 

机构地区：[1]湖南省作物种质创新与资源利用重点实验室/湖南农业大学农学院,长沙410128

出　　处：《作物研究》2013年第3期224-228,共5页Crop Research

基　　金：国家自然科学基金项目(31071674)

摘　　要：利用番茄野生型JAI1和通过不同浓度茉莉酸甲酯(MeJA)处理筛选得到的突变体jai1-1的子叶和下胚轴为材料进行离体组织培养,以探究不同激素组合对愈伤组织的诱导、不定芽分化及生根的影响,最后利用PCR反应验证筛选得到的材料的准确性。结果表明,1/2 MS+50μM/L MeJA是突变体jai1-1筛选的有效培养基。野生型JAI1和突变体jai1-1在MS+6-BA 2.0 mg/L+IAA 0.2 mg/L诱导愈伤组织的效果最好;JAI1在MS+ZT 1.0mg/L+IAA 0.1mg/L诱导分化不定芽的效率最高,而jai1-1则以MS+ZT 2.0 mg/L+IAA 0.2 mg/L为佳;1/2 MS+NAA 0.1 mg/L是两者诱导生根的理想培养基。Tomato（Lycopersicon esculentum Mill ）wild type JAil and mutant jail-1 screened by different concentrations of MeJA were used as the materials in the study. The cotyledon and hypocotyl of wild type JAil and mutant jail-1 were cul- tured in vitro to research the effects of genetype and different hormone combinations on tomato callus induction, adventition bud differentiation and rooting, and then identified the results with PCR reaction. The results show that 1/2 MS ＋ MeJA 50 I^M/L was the proper media to screen the mutant jail-l, and that MS ＋ ZT 1.0 mg/L ＋ IAA 0. 1 mg/L and MS ＋ ZT 2. 0 mg/L ＋ IAA 0. 2 mg/L were the best media for adventition bud differentiation for JAil and jail-1 respectively. MS ＋ 6-BA 2. 0 mg/L ＋ IAA 0. 2 mg/L and 1/2MS ＋ NAA 0. 1 mg/L were the best media for callus induction and root induction for both JAil and jail-1, respectively.

关 键 词：番茄 组织培养 愈伤组织 再生体系 

分 类 号：Q813.12[生物学—生物工程] S641.2[农业科学—蔬菜学]