复方仙草颗粒对IgA肾病大鼠转化生长因子β_1及其Ⅱ型受体表达的影响  

Effect of Compound Xiancao Granules on Expression of TGF-β_1 and TβR-Ⅱ in Renal Tissue among Rats with IgA Nephropathy

作　　者：向少伟[1] 黄国东[1] 黄仁发[1] 贺西南[1] 赖申昌[1] 许健[2] 

机构地区：[1]广西中医药大学附属瑞康医院肾内科,广西南宁530011 [2]浙江省衢州市人民医院中医科,浙江衢州324000

出　　处：《安徽中医学院学报》2013年第4期65-69,共5页Journal of Anhui Traditional Chinese Medical College

基　　金：广西壮族自治区自然科学基金(0991171);广西科学研究与技术开发项目(0719006-3-6);广西壮族自治区教育厅科研项目(200810MS022)

摘　　要：目的观察复方仙草颗粒对IgA肾病大鼠转化生长因子-β1(transforming growth factorβ1,TGF-β1)信号通路的影响。方法采用一侧肾切除、反复尾静脉注射葡萄球菌肠毒素B的方法,复制IgA肾病大鼠模型。将大鼠随机分为模型组,福辛普利组,复方仙草颗粒小、中、大剂量组,另设正常对照组,每组10只。治疗8周后检测24h尿蛋白、尿红细胞计数,采用RT-PCR法检测肾组织TGF-β1及其Ⅱ型受体(TβRⅡ)mRNA的表达,采用Western印迹法检测TGF-β1及TβRⅡ蛋白的表达,采用免疫组织化学法检测纤连蛋白(fibronectin,FN)的表达。结果与正常对照组比较,模型组24h尿蛋白,尿红细胞计数,TGF-β1、TβRⅡmRNA及其蛋白表达水平,以及FN表达水平显著升高(P<0.01);复方仙草颗粒能够剂量依赖性地降低上述指标的水平,其中复方仙草颗粒大剂量组上述指标的水平显著低于小剂量组(P<0.05,或P<0.01),复方仙草颗粒大剂量组在降低24h尿蛋白,TGF-β1mRNA及其蛋白,以及FN方面与福辛普利组比较,差异无统计学意义(P>0.05)。结论复方仙草颗粒能延缓IgA肾病肾小球硬化,减轻血尿、蛋白尿,其作用机制可能与抑制TGF-β1信号通路有关。Objective To investigate the effect of Compound Xiancao Granules （containing Mesona chinensis） on the expression of transforming growth factor-β1 （TGFβ1） and TGF-β type II receptor （TβR- II ） in renal tissue among rats with IgA nephropathy （IgAN）. Methods Sixty rats were randomly and equally divided into normal control group, model group, fosinopril group, and low-, middle-, and high-dose Compound Xiancao Granule groups. In the model group, fosinopril group, and Compound Xiancao Granule groups, a rat model of IgAN was induced by unilateral nephrectomy and repeated injection of staphylococ- cal enterotoxin B into the caudal vein. Then, these groups received respective treatments. Eight weeks lat er, 24 h urinary protein excretion and urinary erythrocyte count were measured; the mRNA and protein expression of TGF-β1 and TβR-II in renal tissue was measured by RT-PCR and Western blot; the expression of fibronectin （FN） in renal tissue was determined by immunohistochemistry. Results Compared with the normal control group, the model group had significant increases in 24-h urinary protein excretion, urinary erythrocyte count, mRNA and protein expression of TGF-β1 and TβR- II , and FN expression （P〈0.01）. Compound Xiancao Granules decreased the above indices in a dose dependent manner, and these indices were significantly lower in high dose Compound Xiancao Granule group than in low-dose Compound Xiancao Granule group （P〈0.05 or P〈0.01）. The decreases in 24 h urinary protein excretion, TGFβ1 mRNA and protein, and FN showed no significant differences between the high dose Compound Xiancao Granule group and fosinopril group （P〈0.05）. Conclusion Compound Xiancao Granules can delay glo- merular sclerosis and reduce hematuria and proteinuria in rats with IgAN. The mechanism may be related to their inhibitory effect on TGF-β1 signaling pathway.

关 键 词：IGA肾病 复方仙草颗粒 转化生长因子Β1 转化生长因子βⅡ型受体 

分 类 号：R692.3[医药卫生—泌尿科学]