SELDI技术在评估慢性病患者焦虑情绪中的应用  

Application of SELDI in evaluating chronic patients＇ anxious emotions

作　　者：朱林[1] 侯向华[1] 徐丽[1] 王清馨[2] 

机构地区：[1]济宁医学院附属医院护理部,272029 [2]山西省肿瘤医院肿瘤防治办公室,太原030013

出　　处：《中华现代护理杂志》2013年第18期2122-2126,共5页Chinese Journal of Modern Nursing

基　　金：山西省归国留学生基金项目（200571）

摘　　要：目的探讨慢性症患者焦虑的发生情况及在焦虑状态下其血清焦虑蛋白质指纹图谱的变化规律。方法采用自身对照研究方法随机选取慢性症患者30例，于住院当天进行焦虑自评量表（SAS）评估，次日抽静脉血行蛋白质飞行时间质谱技术（SELDI—TOF-MS）检测；根据SAS和SELDI结果进行4周心理干预，比较干预前后SAS评分和焦虑蛋白质指纹图谱变化。结果30例慢性病患者干预前SAS评分为（47．30±5．541）分，心理干预后SAS评分降为（33．73±7．887）分，差异有统计学意义（t=7．709，P〈0．01）；干预前SELDI检测焦虑蛋白阳性率50．0％，干预后无一例阳性，干预前后蛋白质指纹图谱比较差异有统计学意义（X。=20．00，P〈0．01）；慢性病患者血清蛋白质指纹图谱：在慢性病患者SAS评分结果显示无焦虑时血清中无蛋白质指纹图谱出现，在质荷比（M／Z）15000＋H-16800＋H之间无任何峰簇出现，丰度〈5％，焦虑相关蛋白指纹（-）；焦虑时血清中捕获到蛋白质指纹图谱，在质荷比（M／Z）15000＋H-16800＋H之间出现一组单峰或双峰质谱峰簇（cluster），丰度≥5％，焦虑相关蛋白指纹（＋）；无焦虑时部分血清中仍然会捕获到蛋白质指纹图谱，在质荷比（M／Z）15000＋H～16800＋H之间出现一组单峰或双峰质谱峰簇（cluster），丰度≥5％，焦虑相关蛋白指纹（＋）。结论慢性病患者焦虑的发生率相对较高，在用SELDI技术获取的蛋白质指纹图谱上，在质荷比（M／Z）15000＋H-16800＋H之间出现一组单峰或双峰质谱峰簇（cluster），其最大丰度≥20％，最小丰度≥5％，其上游与下游之间内无任何质谱峰簇出现，经心理干预或疏导后该质谱峰簇消失。Objective To discuss the incidence of chronic patients＇ anxiety and the variation of serum proteins fingerprints spectrum under anxiety. Methods 30 patients with chronic diseases were randomly selected, SAS was used to evaluate on the first day of admission, and SELDI-TOF-MS was used on the second day. 4 weeks of psychological intervention was implemented according the SAS and SELDI results, SAS score and variation of serum proteins fingerprints spectrum were compared before and after intervention. Results SAS score in 30 patients was （47.30±5. 541 ） before intervention and （33.73±7. 887） after intervention, and the difference was statistically significant （t =7. 709,P 〈0.01 ）. The positive rate of anxiety protein was 50.0% before intervention and non after, and its variation has statistically significant difference （ X： = 20.00, P 〈 0.01 ）. There was no proteins fingerprints spectrum when SAS showed no anxiety, no cluster between 15 000 ＋ H -16 800 ＋ H, abundance 〈 0. 5% and anxiety related protein fingerprint （ - ）. There was proteins fingerprints spectrum during anxiety, a group of tmimodal or two - humped cluster between 15 000＋ H - 16 800 ＋ H, abundance ≥5% and anxiety related protein fingerprint （ ＋ ）. There was still proteins fingerprints spectrum in serum when SAS showed no anxiety, a group of unimodal or two - humped cluster between 15 000 ＋ H - 16 800 ＋ H, abundance≥5% and anxiety related protein fingerprint （ ＋ ）. Conclusions Patients with chronic diseases have relatively higher rate of anxiety. There is a group of unimodal or two-humped cluster between 15 000 ＋ H - 16 800 ＋ H in proteins fingerprints spectrum by SELDI, with the maximum abundance ≥20% and minimums〉5% and no clusters between upper and lower reaches. Clusters disappear after psychological intervention or counseling.

关 键 词：慢性病 焦虑 SELDI 心理干预 

分 类 号：R4[医药卫生—临床医学] R74