热应激预处理对提高小鼠抗缺氧能力的研究  

Effect of Whole-body Heat Stress Preconditioning on Resistance to Hypoxia in Rat

作　　者：徐安聪[1] 蒋洁[2] 崔立静[1] 符永帅[1] 蔡乌妹[1] 施子旭[1] 王丹妹[3] 

机构地区：[1]海南医学院 [2]海南医学院理学院病理教研室 [3]海南医学院理学院机能实验室

出　　处：《中华全科医学》2013年第9期1332-1333,共2页Chinese Journal of General Practice

基　　金：海南省自然科学基金资助项目(811203);海南省教育厅项目(Hjk2012-31)

摘　　要：目的观察全身热应激预处理对提高小鼠抗缺氧能力的作用及探讨其可能机制。方法通过传统的空白对照实验：即74只雄性小鼠随机分为丙组，实验组37只，对照组37只。实验组为热应激预处理（当小鼠肛温达到40～41℃后维持该温度区间10min）＋抗缺氧实验I在缺氧瓶中加入钠石灰（铺满瓶底即可），将实验组小鼠放入缺氧瓶中，盖紧瓶塞]；对照组为不做任何预处理＋抗缺氧实验。记录比较两组小鼠在缺氧环境中的存活时间。实验结果采用SPSS10．0统计软件进行分析比较显著性。结果实验组小鼠存活时间为（28．46±7．60）min；对照组存活时间为（20．03±4．77）min，P〈0．001，实验组小鼠存活时间比对照组长。结论热应激预处理对提高小鼠抗缺氧能力有一定作用，可能与热应激蛋白清除氧自由基减少氧自由基对细胞损伤有关。Objective To observe the effects of whole-body heat stress preconditioning on enhancing the resistance to hypoxia in rat and explore its possible mechanisms. Methods A controlled experiment was designed ：74 male mice were randomly divid- ed into 2 groups, the experimental group （ n = 37 ） and control group （ n = 37 ）. The experimental group received the heat stress pre- conditioning（ the temperature was maintained for 10 minutes when the rectal temperature rose to 40-41 ℃ ） and anti-hypoxia ex- periment（ sodium lime was added to the bottle to form a hypoxia environment,the experimental mice were placed into the bottle, the stopper was covered tightly）. The control group did not receive the heat stress preconditioning. The survival time of mice in hypoxia environment were compared and analyzed by using SPSS 10. 0 statistical software. Results The survival time was 28.46 ± 7.60 in the experimental group and 20.03 ± 4.77 in the control group, P 〈 0. 001. Conclusion The whole-body heat stress preconditioning can improve the resistance to hypoxia in mice ; this may be associated with the functions of heat stress protein on scavenging oxygen free radicals and reducing the injury of oxygen free radicals on cells.

关 键 词：热应激预处理 氧自由基 抗缺氧 

分 类 号：R594.1[医药卫生—内科学] R341.9[医药卫生—临床医学]