羧甲基琼脂糖微球的制备及其在国产碱性蛋白酶纯化中的应用  

Preparation of carboxymethyl agarose and its application in the domestic alkaline protease purification

作　　者：刘微[1,2] 王勇尊[1,2] 许慧[1] 李优鑫[1] 包建民[1,2] 

机构地区：[1]天津市现代药物传递及功能高效化重点实验室,天津大学药物科学与技术学院,天津300072 [2]天津奥顺达科技有限公司,天津300452

出　　处：《现代化工》2013年第7期72-76,共5页Modern Chemical Industry

基　　金：国家自然科学基金资助项目(21075088);天津大学自主创新基金资助项目(1102213)

摘　　要：以琼脂糖粉末为原料,利用反相悬浮原理制备琼脂糖凝胶微球。所得琼脂糖微球的粒径分布在45～165μm范围内,并对其进行了交联。以自制交联琼脂糖微球为基质,对其进行了羧甲基化反应。依据离子交换容量进行键合量的表征,综合考察了各种反应试剂的用量、反应温度、反应时间等条件对键合量的影响。将所得的羧甲基键合微球用于碱性蛋白酶粗品的分离纯化,结果表明,自制羧甲基弱阳离子交换填料对国产碱性蛋白酶具有较好的分离纯化、除色以及除味等作用,可以达到国外同类产品的效果。By using agarose as the raw material,agarose spheres are prepared based on the reverse suspension principle.The particle sizes of those spheres range from 45μm to 165μm.The obtained agarose spheres can be further cross-linked for enhanced mechanical strength,which can be further derivatized with carboxymethyl agents.The effects of reagent amount,the reaction temperature and time are performed to maximize ion exchange capacity.The final carboxymethyl containing agarose spheres are used for the separation and purification of crude alkaline protease and show comparable efficiency with the similar foreign products.

关 键 词：琼脂糖凝胶微球 羧甲基琼脂糖微球 弱阳离子交换填料 碱性蛋白酶 

分 类 号：TQ316.2[化学工程—高聚物工业]