芦荟大黄素对高转移乳腺癌细胞MDA-MB-231体外转移潜能的影响  

Effect of Aloe emodin on metastatic abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro

作　　者：何振辉[1] 何太平[2] 翁闪凡[1] 黄越群[1] 梁念慈[3] 

机构地区：[1]佛山科学技术学院医学院医学检验系,广东佛山528000 [2]广东医学院生物化学与分子生物学研究所,广东湛江524023 [3]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《中国药理学通报》2013年第8期1114-1118,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 39870900);佛山市医学类科技攻关项目(No 201108064);广东省中医药局建设中医药强省课题(No 20111057);佛山科学技术学院博士启动基金(No 2012006)

摘　　要：目的研究芦荟大黄素(Aloe emodin,AE)对人高转移乳腺癌细胞MDA-MB-231体外转移潜能的影响及其作用机制。方法 MTT法检测AE对MDA-MB-231细胞增殖的抑制作用;Transwell chamber法检测AE对MDA-MB-231细胞侵袭重组基底膜能力和趋化性运动能力的影响;RT-PCR、Western blot法检测AE对MDA-MB-231细胞黏着斑激酶(FAK)mRNA和蛋白表达的影响。明胶酶谱法检测MDA-MB-231细胞分泌的基质金属蛋白酶-9(MMP-9)活性。结果 80μmol·L-1 AE抑制MDA-MB-231细胞体外侵袭重组基底膜能力、趋化性运动能力,其抑制率分别为(52.98±5.46)%,(45.88±8.51)%。作用于MDA-MB-231细胞24 h后,AE下调FAK mRNA和蛋白表达,下调MDA-MB-231细胞分泌MMP-9。结论 AE抑制MDA-MB-231细胞体外侵袭能力、趋化性运动能力,其作用机制与其下调FAK表达和MMP-9的分泌有关。Aim To investigate the effect of Aloe emodin （AE） on metastasis-associated abilities of human high metastatic breast cancer MDA-MB-231 cells in vitro. Methods MTF assay was used to evaluate the cell viability of MDA-MB-231 cells after 6h AE treatment. The effect of AE on the invasion and migration of MDA-MB-231 cells was determined by transwell chamber assay. RT-PCR, Western blot were applied to detect the expression levels of FAK mRNA and protein in MDA-MB-231 cells. The secretion of matrix metal- loproteinase-9 （ MMP-9 ） from MDA-MB-231 cells was detected by zymography assay after AE treatment. Results AE significantly inhibited the invasion and migration of MDA-MB-231 cells in vitro, the inhibitory rates were（52.98 ±5.46）%, （45.88±8.51）% after 6h treatment with 80 μmol · L-1 AE. After 24h treatment, AE down-regulated the expression levels of FAK mRNA and protein of MDA-MB-231 cells. The secretion of MMP-9 from MDA-MB-231 cells was inhibited by 24h AE treatment. Conclusion AE can inhibit the invasion and migration of MDA-MB-231 cells. Its possible mechanism may be involved in the deduction of the expression level of FAK and the production of MMP-9.

关 键 词：芦荟大黄素 侵袭 转移 黏着斑激酶 基质金属蛋白酶-9 乳腺癌 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]