白芍总苷对腹膜纤维化大鼠转化生长因子-β1的影响  

Effect of Total Glucosides of Paeony on Transforming Growth Factor Beta 1 in Peritoneal Fibrosis Rats

作　　者：张奡[1,2] 汤水福[3] 练建红 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学附属广东省第二中医院肾内科,广东广州510095 [3]广州中医药大学第一附属医院肾内科,广东广州510405 [4]广州市中医院,广东广州510013

出　　处：《广州中医药大学学报》2013年第4期511-514,607,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金资助项目(编号:S2011010005292)

摘　　要：【目的】探讨白芍总苷(the total glucosides of paeony,TGP)对腹膜纤维化大鼠转化生长因子-β1(TGF-β1)的影响。【方法】选用健康SD雄性大鼠32只,随机分为4组,每组8只,分别为空白组、模型组、白芍总苷高剂量组、白芍总苷低剂量组。采用腹腔注射42.5 g/L含糖透析液+脂多糖法复制腹膜纤维化模型。白芍总苷高、低剂量组造模同时给予白芍总苷灌胃(剂量分别为200、100 mg.kg-1.d-1);实验第30天结束后留取腹膜组织和腹透液,采用一步法Real-time PCR测定腹膜组织TGF-β1 mRNA的表达,采用酶联免疫吸附(ELISA)法测定腹透液中TGF-β1的水平。【结果】模型组腹膜TGF-β1mRNA的表达及腹膜透析液TGF-β1的水平显著高于空白组(P<0.001),白芍总苷高、低剂量组2指标水平较模型组均显著降低(P<0.05)。【结论】白芍总苷可以上调TGF-β1的表达,对腹膜纤维化具有一定防治作用。Objective To explore the effect of the total glucosides of paeony（TGP） on transforming growth factor beta 1（TGF-β1） in peritoneal fibrosis rats.Methods Thirty-two healthy SD male rats were equally randomized into 4 groups,and they were blank control group,model group,high-dose and low-dose TGP groups.Rat model with peritoneal fibrosis was induced by intraperitoneal injection of 42.5 g/L high-glucose dialysate combined with lipopolysaccharide（LPS）.The rats in TGP groups were given gastric gavage of 100,200 mg·kg^-1·d^-1 TGP respectively during the modeling.On experiment day 30,all rats were sacrificed,and peritoneal tissue and peritoneal dialysis fluid were sampled.One-step real-time polymerase chain reaction（PCR）was used to examine TGF-β1 mRNA in the visceral peritoneum,and TGF-β1 level in the peritoneal dialysis fluid was detected by enzyme-linked immunospecific assay（ELISA）method.Results TGF-β1 mRNA expression and TGF-β1 level were increased in the model group than those in the blank control group（P〈0.001）,and were decreased in high-dose and low-dose TGP groups（P〈0.05 compared with the model group）.Conclusion TGP can decrease the expression of TGF-β1 in the peritoneum,and show certain preventive and therapeutic effect on peritoneal fibrosis.

关 键 词：腹膜纤维化 中药疗法 白芍总苷 药理学 TGF-Β1 基因表达调控 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]