益气除痰方对小鼠Lewis肺癌细胞凋亡形态学的影响  

Effect of Yiqi Chutan Recipe on Apoptosis of Lewis Lung Carcinoma Cells in Mice

作　　者：杨建猛[1] 林丽珠[1,2] 方若鸣[1] 杨文娟[1] 孙玲玲[1,2] 

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广州中医药大学第一附属医院肿瘤科,广东广州510405

出　　处：《广州中医药大学学报》2013年第4期533-536,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金自然申请项目(编号:10151040701000021);广东省高等学校高层次人才项目(编号:A1100005)

摘　　要：【目的】探讨益气除痰方对C57BL/6J小鼠Lewis肺癌细胞凋亡形态学的影响。【方法】选用C57BL/6J小鼠,随机分成中药组(益气除痰方组,剂量为27.3 g.kg-.1d-1)、顺铂组(剂量为1 mg.kg-.1d-1)、联合组(中药联合顺铂)、模型组,采用右腋部皮下接种Lewis肺癌细胞株法造模;采用流式细胞术测定各组小鼠Lewis肺癌细胞的凋亡率及通过透射电镜观察Lewis肺癌细胞超微结构的变化。【结果】中药组、顺铂组、联合组的细胞凋亡率均显著升高,与模型组比较差异有统计学意义(P<0.01或P<0.001);中药组、顺铂组、联合组在透射电镜下均可观察到Lewis肺癌细胞呈现早期凋亡形态。【结论】益气除痰方治疗肺癌的作用与诱导肺癌细胞凋亡有关。Objective To study the effect of Yiqi Chutan Recipe（YCR） on apoptosis of Lewis lung carcinoma cells in C57BL/6J mice.Methods C57BL/6J mice were randomized into YCR（27.3 g·kg^-1·d^-1）group,Cisplatin（1 mg·kg^-1·d^-1）group,combination group（YCR 27.3 g·kg^-1·d^-1 plus Cisplatin 1 mg·kg^-1·d^-1））,and model group.The mice models were established by subcutaneous inoculation of Lewis lung carcinoma cells into the right axillary fossa.Apoptotic rate of Lewis lung carcinoma cells in each group was determined by flow cytometry,and the ultrastructure of Lewis lung carcinoma cells was observed under transmission electron microscope.Results The apoptotic rate was obviously increased in YCR group,Cisplatin group and the combination group（P0.01 or P0.001 compared with model group）.Early apoptotic features were shown in Lewis lung carcinoma cells of the three medication groups under transmission electron microscope.Conclusion The therapeutic mechanism of YCR for lung cancer is probably related with the induction of Lewis lung carcinoma cells apoptosis.

关 键 词：益气除痰方 药理学 肺癌 中药疗法 细胞凋亡 肺癌细胞 超微结构 流式细胞术 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]