纤维素组合酶在不同品种生长猪饲粮中的适宜添加量  

Appropriate Addition of Combining Cellulase in Diets for Growing Pigs of Different Breeds

作　　者：耿梅[1] 姜建阳[1] 韩先杰[1] 宋春阳[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《动物营养学报》2013年第7期1541-1550,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省良种工程重大课题项目(2009LZ012-04);山东省现代农业产业技术体系生猪创新团队资助(SDNY201226)

摘　　要：本试验利用体外消化试验筛选出3种纤维素酶的最优组合,采用消化代谢试验来确定所筛选的纤维素组合酶在2个品种猪纤维性饲粮中的适宜添加量。试验以酸性洗涤纤维(ADF)为评定指标,以地瓜蔓为粗纤维来源,配制3.8%ADF和5.8%ADF的2种纤维水平的饲粮。分别选取5头体重[(25±1)kg]相近、健康去势的烟台黑猪和杜×鲁烟白杂交猪,于回肠末端安装T型瘘管,设5个最优纤维素组合酶添加量:0(对照组)、0.05%、0.10%、0.15%、0.20%,采用5×5拉丁方设计进行消化代谢试验。结果表明:在5.8%ADF水平饲粮中,与对照组相比,纤维素组合酶添加量分别为0.10%和0.15%时,对2个品种猪养分消化率以及氮平衡的影响达到了显著水平(P<0.05),但在3.8%ADF水平饲粮中差异不显著(P>0.05)。在5.8%ADF水平饲粮中,在纤维素组合酶添加量相同的情况下,对于必需氨基酸,蛋氨酸、缬氨酸、异亮氨酸、苯丙氨酸的回肠表观消化率烟台黑猪极显著高于杜×鲁烟白杂交猪(P<0.01);对于非必需氨基酸,除丝氨酸和甘氨酸回肠表观消化率差异不显著(P>0.05)外,其他氨基酸回肠表观消化率差异极显著(P<0.01)。结果提示,在5.8%ADF水平饲粮中,烟台黑猪纤维素组合酶适宜添加量为0.10%,杜×鲁烟白杂交猪为0.15%。This test was used an in vitro digestion test to screen the optimal combination of three kinds of cellulase, and aimed to study the appropriate addition of combining cellulase in diets for growing pigs of different breeds. The test used acid detergent fiber （ADF） as an evaluation index and prepared 3.8% ADF and 5.8% ADF of dietary fiber with sweet potato vine. Five Yantai Black growing pigs and five Duroc？Luyan White hybrid pigs with an average body weight of （25？1） kg were used, and were installed T fistulas in the terminal ileum. A 5 ？ 5 Latin square design was used in digestibility test, and the adding levels of combining cellulase were 0 （control group）, 0.05%, 0.10%, 0.15% and 0.20%, respectively. The results showed as follows： the combining cellulase in the diets with 5.8% ADF significantly promoted nutrient digestibility and nitrogen balance in growing pigs of different breeds （P〈0.05）, but no significant difference was found in the diets with 3.8% ADF （P〉0.05）. Under the condition of the diets with 5.8% ADF and with the same addition of combining cellulose, for the essential amino acid, including Met, Val and Phe, the ileal apparent digestibility of Yantai Black pigs was significantly higher than that of Duroc？Luyan White hybrid pigs （P〈0.01）; for the non-essential amino acids, the ileal apparent digestibility of Ser and Gly was not significant （P〉0.05）, and that of the other amino acids was significant difference （P〈0.01）. The appropriate addition of combining cellulase in diets with 5.8% ADF was 0.10% for Yantai Black growing pigs and 0.15% for Duroc？Luyan White hybrid pigs.

关 键 词：纤维素酶 烟台黑猪 杜×鲁烟白杂交猪 养分消化率 氮平衡 氨基酸回肠表观消化率 

分 类 号：S828[农业科学—畜牧学]