MIF基因多态性与子宫内膜异位症的相关性  

Association of macrophage migration inhibitory factor gene polymorphisms with endometriosis

作　　者：于雅[1] 袁清连[1] 王春平[1] 刘建平[2] 

机构地区：[1]深圳市罗湖区人民医院妇二科,广东深圳518001 [2]深圳市疾病预防控制中心营养与食品卫生科,广东深圳518055

出　　处：《中国热带医学》2013年第5期625-628,共4页China Tropical Medicine

基　　金：深圳市罗湖区科技计划项目(No.200902166)

摘　　要：目的研究巨噬细胞移动抑制因子(Macrophage migration inhibitory factor,MIF)+254 T/C(rs2096525)及-173G/C(rs755622)基因多态性与子宫内膜异位症易感性的关系。方法自2009年6月至2011年12月收集病理学确诊的子宫内膜异位症患者112例为病例组,另选取同期非子宫内膜异位症者112例为对照组。采用聚合酶链-限制性片段长度多态性(PCR-RFLP)对MIF基因多态性进行分型,分析两组MIF基因型的分布及MIF基因多态性与子宫内膜异位症发病风险的关系。结果 MIF基因+254 T/C多态性中病例组和对照组中CC基因型频率分别为7.14%和1.79%,两组比较差异有统计学意义(P=0.04)。两组C等位基因频率分别为23.66%和14.29%,差异有统计学意义(P=0.01);以TT基因型为参照,病例组携带CC基因型是对照组发生子宫内膜异位症危险的4.89倍,95%可信区间为1.01-23.83;且携带C突变基因的个体发生子宫内膜异位症的OR及其95%可信区间为1.86(1.15-3.02)。病例组和对照组间MIF-173G/C各GG基因型频率和等位基因频率差异均没有统计学意义(P>0.05);结论 MIF基因+254 T/C多态性与子宫内膜异位症发生的易感性有关,C等位基因可能是其发生的遗传易感标记。Objective To investigate correlation polymorphisms of macrophage migration inhibitory factors（MIF）（rs755622 and rs2096525）with susceptibibility of endometriosis in a Han population in Guangdong province.Methods The 112 patients with endometriosis were selected from Shenzhen Municipal Fifth People’s Hospital from June 2009 to December 2011.The cases without endometriosis in the same period Were raken ascontrols.The genotypes of the patients in both groups were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis（PCR-RFLP）.Then the differences of genotype frequencies and the allele frequencies in each group were compared and the association of MIF gene polymorphisms with the endometriosis was explored.Results The distribution frequencies of CC genotype of＋254（T/C）locus in MIF gene were 7.14% and 1.79%,respectively.There were statistical differences in genotype frequencies between the endometriosis group and the control group（P=0.04）;The frequencies of C allele of＋254（T/C）locus in MIF gene were 23.66% and 14.29%,respectively.Showing significant difference in frequencies between the two groups（P=0.01）.OR values（95%CI）with CC genotypes were 4.89（1.01-23.83）.OR value（95%CI）for subjects with T allele was1.86（1.15-3.02）.There were no statistical differences in genotype frequencies and allele frequencies of-173 locus in MIF gene between two groups（P〈0.05）.Conclusions The results suggest that MIF ＋254 T/C polymorphism is significantly correlated with susceptibility of endometriosis,Callele and CC homozygote may be risk factors for endometriosis.

关 键 词：子宫内膜异位症 巨噬细胞移动抑制因子 基因多态性 

分 类 号：R711.71[医药卫生—妇产科学]