miRNA144-3p和miRNA146a-5p在结核性胸膜炎患者外周血中的表达  

The expression level of miRNA144- 3p and miRNA146a- 5p in the periphery blood of patients with tuberculosis pleurisy

作　　者：张帆 刘守江 魏巍 覃林珍 闫敬 王三清 王健 

机构地区：[1]深圳市南山区慢性病防治中心结核病防治科,广东深圳51805

出　　处：《中国热带医学》2013年第6期664-666,共3页China Tropical Medicine

基　　金：深圳市科技计划项目资助(No.201203232)

摘　　要：目的探讨miRNA144-3p和miRNA146a-5p在结核性胸膜炎患者外周血表达及其临床意义。方法收集住院初治结核性胸膜炎患者、结核潜伏感染者和健康对照各30例。采集外周血提取RNA,采用荧光定量PCR技术检测miRNA144-3p和miRNA146a-5p在各组患者外周血中的表达差异。结果结核性胸膜炎患者外周血组miRNA144-3p表达显著低于结核潜伏感染者和健康对照外周血组(P<0.05),而结核潜伏感染者和健康对照外周血组之间差异无统计学意义。miRNA146a-5p表达在三组之间差异均无统计学意义(P>0.05)。结论miRNA144-3p可能参与结核性胸膜炎的发生、发展,且可能对结核性胸膜炎诊断有一定的临床意义。Objective To investigate the expression level of miRNA144-3p and miRNA146a-5p in the periphery blood of patients with tuberculous pleurisy and explore its clinical significance.Methods The expression level of miRNA144-3p and miRNA146a-5p were measured by qRT-PCR in 30 patients with tuberculous pleurisy,Latent tuberculosis infection（LITB） and healthy donors respectively.The statistical difference of the miRNA144-3p and miRNA146a-5p level.In each groups were analyzed.Results The expression level of miRNA144-3p in patients with tuberculous pleurisy were significantly lower than that of the LITB and healthy donors,respectivly（P 0.05） and moreover,no statistic difference was shown between LITB and healthy donors（P 0.05）.Furthermore,the expression levels of miRNA146a-5p in the three groups showed no statistically significant difference（P 0.05）..Conclusion The miRNA144-3p may be a new biomarker in diagnosis of tuberculous pleurisy.

关 键 词：结核 胸膜炎 微RNAS 基因表达 

分 类 号：R521.7[医药卫生—内科学]