后穹窿穿刺液ELISPOT法在盆腔结核诊断中的应用  

Usage of Posterior Fornix Succus ELISPOT as A Diagnostic Method for Pelvic TB

作　　者：杨富强[1,2] 刘焰[1] 

机构地区：[1]武汉大学医学院医学免疫系,武汉430071 [2]襄阳市第一人民医院,湖北襄阳441000

出　　处：《现代检验医学杂志》2013年第3期149-151,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨后穹窿穿刺液酶联免疫斑点试验（ELISPOT）法在盆腔结核诊断中的应用价值.方法 采集64例确诊为盆腔结核患者及56例妇科盆腔恶性肿瘤患者（对照组）后穹隆穿刺液,分离其中单个核细胞采用TB-ELISPOT法检测其结核多肽抗原特异性的T淋巴细胞反应,同时对后穹窿穿刺液采用荧光定量PCR法检测TB-DNA.根据检测结果比较两种方法的灵敏度及特异度.结果 后穹窿穿刺液TB-ELISPOT法及PCR法检测灵敏度分别为92.8%,64.1%,两种方法比较差异有统计学意义（χ^2=14.8,P〈0.01）;两种方法检测特异度分别为92.8%,85.7%,两者相比较,差异无统计学意义（χ^2=1.49,P〉0.05）.结论 采用后穹窿穿刺液检验,并使用TB-ELISPOT法进行结核分枝杆菌检测灵敏度高、特异度好,对于盆腔结核的诊断有很好的应用价值.Objective To investigate the application of ELISPOT （enzyme linked immunospot Assay） in the diagnosis of pel- vic TB. Methods TB-ELSPOT assays were performed in posterior fornix succus mononuclear cell isolated from patients who had already been clinically diagnosed as pelvic TB infection （64 cases） or non-TB infection（56 cases）. The other diagnostic method was TB-DNA assays with RTFQ PCR. Then sensitivities and specificities of results were compared. Results The data of TB-ELISPOT and PCR showed the sensitivity were 92.8 ~ and 64. 1% respectively,and the difference was, sig- nificant respectively （Zz = 14. 8, P〈0. 01）. The specificities were 92. 8 ~ and 85.7 ~ respectively, and the difference was not significant （x^2 = 1.49, P〉0.05）. Conclusion Posterior fornix succus ELISPOT is a specific and sensitive approach in detecting pelvic TB infection.

关 键 词：后穹窿穿刺 酶联免疫斑点试验 盆腔结核 诊断 

分 类 号：R527[医药卫生—内科学] R392.11[医药卫生—临床医学]