N-acetylcysteine protect lymphocytes and cytokines against heavy ion irradiation via counteracting the glutamate  

作　　者：XIE Yi ZHANG Hong WANG Yipei LIU Yang CHEN Yingtai 

机构地区：[1]Institute of Modern Physics,Chinese Academy of Sciences [2]Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences [3]Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province [4]Department of Chemistry & Biochemistry,University of Delaware [5]Department of Abdominal Surgical Oncology,Cancer Institute and Hospital,Chinese Academy of Medical Sciences

出　　处：《Nuclear Science and Techniques》2013年第3期39-44,共6页核技术（英文）

基　　金：Supported by National Basic Research Program(973Program)(No.2010CB834202);National Natural Science Foundation of China(No.10835011);Key Scientific Technology Research Projects of Gansu Province(Nos.0702NKDA045 and 0801NKDA001);West Light Foundation(Doctor aid,2009)

摘　　要：We evaluated the protective effect of N-acetylcysteine (NAC) on immunity system irradiated by 12C6+ ion beam. Kun-Ming mice were whole-body irradiated by 12C6+ ion at doses of 0, 0.5, 1, 1.5, 2, 2.5 and 3 Gy. The results showed that in saline group, the lymphocytes DNA double-strand breaks (DSBs), maleic dialdehyde, thymocytes number in G 0 /G 1 and apoptosis percentage increased with dose increment, and the levels of interferon-γ, glutathione, superoxide radical (SOD) and natural killer cells activity decreased with dose increment. However, there were no significant changes in NAC-treated group. The data indicated that pre-treatment with NAC could significantly remove the ROS by counteracting the glutamate, decrease excessive lipid peroxidation reaction and SOD damages, and protect DNA, lymphocytes and cytokines against irradiation.We evaluated the protective effect of N-acetylcysteine （NAC） on immunity system irradiated by ^12C^6＋ ion beam. Kun-Ming mice were whole-body irradiated by ^12C^6＋ ion at doses of 0, 0.5, 1, 1.5, 2, 2.5 and 3 Gy. The results showed that in saline group, the lymphocytes DNA double-strand breaks （DSBs）, maleic dialdehyde, thymocytes number in Go/G1 and apoptosis percentage increased with dose increment, and the levels of interferon-y, glutathione, superoxide radical （SOD） and natural killer cells activity decreased with dose increment. However, there were no significant changes in NAC-treated group. The data indicated that pre-treatment with NAC could significantly remove the ROS by counteracting the glutamate, decrease excessive lipid peroxidation reaction and SOD damages, and protect DNA, lymphoeytes and cytokines against irradiation.

关 键 词：N-乙酰半胱氨酸 重离子辐照 细胞因子 保护作用 谷氨酸 DNA双链断裂 超氧化物歧化酶 超氧阴离子自由基 

分 类 号：O571.6[理学—粒子物理与原子核物理] R95[理学—物理]