油茶芽苗砧嫁接口愈合过程解剖学研究  

Study on the Anatomical Structures in Development of the Nurse Seed Grafted Union of Camellia oleifera

作　　者：杨志坚[1] 冯金玲[1] 陈辉[1] 

机构地区：[1]福建农林大学林学院,福建省油茶工程技术研究中心,福州350002

出　　处：《植物科学学报》2013年第3期313-320,共8页Plant Science Journal

基　　金：福建省科技平台建设项目(2010N2001);福建省农业科技重大项目(2010N5006);高等学校博士学科点专项科研基金资助课题(20123515110010);福建省教育基金项目(JA12115)

摘　　要：采用石蜡切片法对油茶(Camellia oleifera)芽苗砧嫁接口的愈合过程进行组织解剖学研究。观察结果表明:(1)在嫁接后的第4天,嫁接口产生隔离层;在嫁接后的第8天,嫁接口的砧木产生愈伤组织;在嫁接后的第16天,嫁接口的接穗产生愈伤组织;在嫁接后的第22天,嫁接口的砧木与接穗连接;在嫁接后的第29天,嫁接口的形成层分化形成;在嫁接后的第35天,嫁接口的愈伤组织维管束形成,接穗连接成功。(2)油茶芽苗砧嫁接部位愈伤组织形成前,芽苗砧木的解剖结构在短时间内加速发育,逐渐与接穗的组织结构相似。(3)嫁接口的形成层和其它薄壁细胞组织均能产生愈伤组织,但形成层是其主要来源。Conventional paraffin sectioning was adopted to conduct anatomical examination of the nurse seed grafted Camellia oleifera union during development.Results showed that at day 4 after grafting,the nurse seed grafting union became the isolation layer;at day 8,the callus in the root stock was observed;at day 16,the callus was found in the scion;at day 22,the root stock connected the scion;at day 29,the nurse seed grafting union formed the vascular cambium;at day 35,vascular bundles was formed,which indicated that the nurse seed grafted seedling survived.Before induction of callus in the nurse seed grafted C.oleifera union,the primary organization structure of the stock accelerated growth and development,and gradually became similar to the structure of the scion.The cambium and other parenchyma cells in the grafted union could all be induced to produce callus,but the cambium was the principal source.

关 键 词：油茶 芽苗砧嫁接 愈合 解剖结构 

分 类 号：S794.4[农业科学—林木遗传育种]