二维斑点追踪成像观察新生猪缺氧缺血性脑病模型受损心肌径向运动  

Evaluation on radial motion of damaged myocardium in newborn pigs model with hypoxic-ischemic encephalopathy using speckle tracking imaging technology

作　　者：乔伟[1] 任卫东[1] 肖杨杰[1] 商聪[1] 李红[2] 王晓明[2] 

机构地区：[1]中国医科大学附属盛京医院超声科,辽宁沈阳110004 [2]中国医科大学附属盛京医院放射科,辽宁沈阳110004

出　　处：《中国医学影像技术》2013年第7期1049-1052,共4页Chinese Journal of Medical Imaging Technology

基　　金：辽宁省科技厅课题(2011225015)

摘　　要：目的应用二维斑点追踪成像技术(STI)评价新生猪缺氧缺血性脑病(HIE)模型损伤心肌径向运动,观察心肌组织病理学变化。方法将18只新生约克猪随机分成对照组(n=8)和模型组(n=10),对模型组行气管插管、低氧通气(6%氧气、94%氮气)及双侧颈动脉夹闭术,对照组仅行气管插管、并给予40%氧气,不予颈动脉夹闭术。建模24h后行超声心动图检查,获取左心室各节段径向应变(SR)及应变率(SrR)曲线,测量曲线峰值,取各水平平均值。测量心率(HR)、左心室射血分数(LVEF)及Tei指数。对脑组织及心肌组织行病理学检测。结果与对照组相比,模型组各水平径向应变及应变率峰值均减低,HR增快,LVEF下降,Tei指数升高(P均<0.05)。模型组心肌细胞肿胀、变性、坏死及凋亡;脑神经胶质细胞肿胀、神经元细胞变性及坏死。结论新生猪HIE模型早期即出现心肌径向运动及心功能减弱。新生猪模型可以复制新生儿并发心肌损伤。Objective To observe the changes of radial motion of the damaged myocardium in newborn pig models with hypoxic ischemic-encephalopathy（HIE） with speckle tracking imaging（STI） technology.Methods Eighteen newborn pigs were randomly divided into control group（n=8） and model group（n=10）.The bilateral carotid arteries were blocked,and nitrogen oxygen atmosphere（6% oxygen and 94% nitrogen） were given mechanically through endotracheal intubation in the model group.In the control group,nitrogen oxygen atmosphere（40% oxygen） were given as the model group,but carotid arteries were not blocked.After 24 h,the peak value of radial strain（SR） and radial strain rate（SrR） of each segment were measured with STI.Then the average of global,base,middle and apex levels were calculated,the heart rate（HR）,left ventricular ejection fraction（LVEF） and Tei index were measured.The pathological slices of the myocardium and brain were observed at last.Results Compared with the control group,the peak value of SR and SrR,LVEF decreased,HR and Tei index increased in the model group（all P0.05）.In the model group,swelling,degeneration,necrosis and apoptosis were found in some myocardial cells,while swelling,degeneration and necrosis were found in some brain glial cells and neuronal cells.Conclusion The radial motion and heart function decreased in the early time of newborn pig HIE models.It is a reliable method to use newborn pigs model of HIE to simulate neonatal HIE with myocardial damage.

关 键 词：缺氧缺血 脑 猪 雏型 斑点追踪成像 心肌损伤 径向应变 

分 类 号：R540.45[医药卫生—心血管疾病] R-332[医药卫生—内科学]