转Bt基因玉米根际土壤及秸秆残体中Cry1Ab蛋白含量动态  

Dynamics of Cry1Ab protein content in the rhizosphere soil and straw debris of transgenic Bt corn

作　　者：李凡[1] 王敏[1,2] 孙红炜[1] 杨淑珂[1] 路兴波[1] 

机构地区：[1]山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南250100 [2]中国科学院寒区旱区环境与工程研究所,兰州730000

出　　处：《应用生态学报》2013年第7期1907-1913,共7页Chinese Journal of Applied Ecology

基　　金：国家转基因生物新品种培育重大专项(2013ZX08012-004)资助

摘　　要：采用ELISA法研究了田间种植条件下转Bt基因玉米MON810生育期根际土壤及还田秸秆中Cry1Ab蛋白的田间残留降解动态,并分别用移动对数模型、指数模型和双指数模型对秸秆分解释放Cry1Ab蛋白的田间降解动态进行拟合,估算了DT50和DT90值.结果表明:Bt玉米不同生育期根际土壤中Cry1Ab蛋白含量差异较大,但总体随生育期的延长而显著降低.收获后地表覆盖和埋入土壤两种秸秆还田方式下,秸秆中Cry1Ab蛋白在土壤中的降解规律基本一致,均呈现前期大量快速降解,中后期极少量稳定降解两个阶段.秸秆还田7 d内,地表覆盖处理的Cry1Ab蛋白降解率均极显著高于埋入土壤处理;10 d时两处理的降解率基本一致,分别为88.8%和88.6%;20 d后,两处理秸秆中Cry1Ab蛋白的降解日趋缓慢;至180 d时仍能检测到少量的Cry1Ab蛋白.3种模型均能较好地反映秸秆中Cry1Ab蛋白的田间降解规律,从相关系数(R)及DT90值与实测值的吻合程度来看,双指数模型最优.By using ELISA test kits, a field investigation was conducted on the degradation dynamics of CrylAb protein in the rhizosphere soil of Bt corn MONS10 at its different growth stages and in the MON810 straws returned into field after harvest. Three models （shift-log model, exponential model, and bi-exponential model） were used to fit the degradation dynamics of the CrylAb protein from the straw debris, and the DT50 and DT90 values were estimated. There existed great differences in the CrylAb protein content in the rhizosphere soil of MON810 at its different growth stages, but overall, the CrylAb protein content was decreased remarkably with the growth of MON810. The degradation of Cryl Ab protein from the straws covered on soil surface and buried in soil showed the same two-stage pattern, i. e. , more rapid at early stage and slow-stable in later period. Within the first week after straw return, the degradation rate of the Cryl Ab protein from the straws covered on soil surface was significantly higher than that from the straws buried in soil. At 10 d, the degradation rate of the Cryl Ab protein from the straws covered on soil surface and buried in soil was basically the same, being 88.8% and 88.6%, respectively. After 20 days, the degradation of CrylAb protein entered slow-stable stage. Till at 180 d, a small amount of CrylAb protein could still be de- tected in the straw debris. All of the three models used in this study could fit the decay pattern of the CrylAb protein from the straw debris in field. By comparing the correlation coefficient （R） and the consistency between the measured and calculated DTgo, bi-exponential model was considered to be the best.

关 键 词：BT玉米 Cry1Ab蛋白 根际土壤 秸秆还田 田间降解 拟合模型 

分 类 号：S513[农业科学—作物学]