天山雪莲SikGLP基因的克隆及表达分析  

Cloning and Expression Analysis of SikGLP Gene in Sasussured involucrata Kar.et Kir

作　　者：刘超[1] 张林华[1] 邱红林[1] 王爱英[1] 胡鸢雷[2] 祝建波[1] 

机构地区：[1]石河子大学农业生物技术重点实验室,石河子832003 [2]北京大学生命科学学院,北京100871

出　　处：《植物研究》2013年第4期461-467,共7页Bulletin of Botanical Research

基　　金：国家转基因重大专项(2011ZX08005-004)

摘　　要：从天山雪莲cDNA文库中分析得到一个类萌发素蛋白基因序列,命名为SikGLP。生物信息学分析表明,该基因包含1个633 bp的完整开放阅读框,编码210个氨基酸。SikGLP分子量为21.6 kDa,理论等电点是6.81,并且是具有信号肽的疏水性蛋白。氨基酸多序列比对发现GLP序列保守性较高,具有的GLP的典型特征。17个物种间的系统发育树可以看出与葡萄GLP进化关系最近。Real time-PCR检测表明,SikGLP基因受低温、甲基紫精和PEG诱导表达,可能在胁迫初期对提高植物防御起作用。A novel germin-like protein gene clone was analyzed from a cDNA library of Sasussured involucrate, named SikGLP. Bioinformatics analysis showed that the SikGLP gene contains a 633 bp open reading frame encoding 210 amino acids with a theoretical molecular mass of 21.6 kDa and an isoelectric point of 6.81. SikGLP was a hydrophobic protein and contained a signal peptide. Multiple sequence alignment of GLP proteins revealed that the GLP gene shares high identities in sequence, and possesses typical characteristics of plant germin-like proteins. Phylogenetic analysis among 17 species indicated that SikGLP was clustered with Vitis vinifera. Real time-PCR analysis showed that the expression of SikGLP gene by low temperature, MV and PEG stresses. It may plays a role in improving resistance capacity of early stress.

关 键 词：天山雪莲 类萌发素蛋白 实时定量PCR 

分 类 号：S567.19[农业科学—中草药栽培]