牡丹种皮黄酮提取及对ABTS自由基清除作用  

Extraction of Total Flavonoids from Peony Episperm and ABTS Radical Scavenging Activity

作　　者：孟庆焕[1,2] 王化[1,2] 王洪政[1,2] 吴薇薇[1,2] 钟晨[1,2] 祖元刚[1,2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]东北林业大学林业生物制剂教育部工程研究中心,哈尔滨150040

出　　处：《植物研究》2013年第4期504-507,共4页Bulletin of Botanical Research

基　　金："十二五"国家科技支撑项目(2011BAD33B0203)

摘　　要：以丹凤牡丹(Paeonia ostii)种皮为原料,采用正交实验法对影响牡丹种皮总黄酮匀浆提取含量的主要因素进行研究分析,考察了乙醇浓度、料液比、匀浆时间、匀浆次数4个因素的影响,筛选出最佳工艺条件,乙醇浓度80%,液料比20:1,匀浆时间4min,匀浆次数2次。并在最佳工艺条件下进行验证实验,得牡丹种皮黄酮提取物含量为52.19mg·g^(-1)。测定了牡丹种皮黄酮对2,2-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)自由基的清除效果,结果表明牡丹种皮黄酮的抗氧化能力强于2,6-二叔丁基-4-甲基苯酚(BHT),为牡丹种皮变废为宝提供了科学依据。The optimum homogenate extraction technique of total flavonoids from Paeonia ostii episperm was studied by orthogonal test. The optimum extraction conditions were obtained as follows： 80% ethanol, ratio of solvent to material 20：1, two times homogenate, and each 4 min. Under this conditions, the content of flavonoids in P. ostii episperm was 52.19 mg ABTS radical scavenging experiment showed that fiavonoids from P. ostii episperm had significant effects, which was higher than BHT, but less than VC. In addition, we have realized the practical process of making waste into useful material.

关 键 词：牡丹种皮 黄酮 ABTS自由基 匀浆法 

分 类 号：S685.11[农业科学—观赏园艺]